青海高原牦牛PRDM16基因克隆、生物信息学及组织差异表达分析

doi:10.16431/j.cnki.1671-7236.2016.02.011

Abstract

Abstract: In this study,the part coding region of PRDM16 gene of the Qinghai plateau yak was cloned and its bioinformatics and differential expression of PRDM16 gene of muscle tissue were analyzed in different sex individual.The PRDM16 gene part of CDS region was cloned from the longissimus dorsi muscle tissue of Qinghai plateau yak,analyzed its characteristics of bioinformatics and detected gene expression levels of different sex individuals in muscle tissue by Real-time PCR.The results showed that the sequence length of the cloned fragment was 323 bp,the homology between Qinghai plateau yak and cattle was 100%.It encoded 99 amino acids,containing domains of MDS1-EVI1 (complex locus protein MDS1) family proteins function,CATH protein function and typical structure of the curly spiral.PRDM16 protein was 20% of the similarity with people methyltransferase protein domain PR protein 1.PRDM16 gene expression of female yak was significantly higher than male yak's in longissimus dorsi muscle tissue (P<0.01).The test results provided not only research foundation for genetic progress but also technical references for yak meat quality analysis.

Key words: Qinghai plateau yak; PRDM16 gene; longissimus dorsi; gene expression

CLC Number:

ZHAO Sheng-jun, ZHANG Yong, GUO Xian. Cloning,Bioinformatics and Tissue Differential Expression Analysis of PRDM16 Gene in Qinghai Plateau Yak[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(2): 363-370.

References

[1] 孔祥颖,张丽,保善科,等.放养过程中补饲对青海高原牦牛产肉能力及肉品质的影响[J].中国畜牧兽医,2015,42(1):104-108.
[2] Huang X D,Tan H Y,Long R,et al.Comparison of methanogen diversity of yak (Bos grunniens) and cattle (Bos taurus) from the Qinghai-Tibetan plateau,China[J].BMC Microbiology,2013,12:237-247.
[3] 杨雪蓉,陈代文,余冰,等.PRDM16的研究进展[J].动物营养学报,2010,22(6):1477-1481.
[4] Hohenauer T,Moore A W.The prdm family:Expanding roles in stem cells and development[J].Development,2012,139(13):2267-2282.
[5] Kajimura S,Seale P,Tomaru T,et al.Regulation of the brown and white fat gen programs through a PRDM16/CtBP transcriptional complex[J].Genes Dev,2008,22(10):1397-1409.
[6] Cristancho A G,Schupp M,Lefterova M I,et al.Repressor transcription factor 7-like 1 promotes adipogenic competency in precursor cells[J].Proc Nati Acad USA,2011,108(39):16271-16276.
[7] Lee J E,Ge K.Transcriptional and epigenetic regulation of PPARγ expression during adipogenesis[J].Cell Biosci,2014,4:29-39.
[8] Chuikov S,Levi B P,Smith M L,et al.PRDM16 promotes stem cell maintenance in multiple tissues,partly by regulating oxidative stress[J].Natural Cell Biology,2010,12(10):999-1006.
[9] Yoshida M,Nosaka K,Yasunaga J,et al.Aberrant expression of the MEL1S gene identified in association with hypomethylation in adult T-cell leukemia cells[J].Blood,2004,103(7):2753-2760.
[10] DiZazzo E,De Rosa C,Abbondanza C,et al.PRDM proteins:Molecular mechanisms in signal transduction and transcriptional regulation[J].Biology,2013,2(1):107-141.
[11] Herz H M,Garruss A,Shilatifard A.SET for life:Biochemical activities and biological functions of SET domain-containing proteins[J].Trends in Biochemical Sciences,2013,38(12):621-639.
[12] Lanouette S,Mongeon V,Figeys D,et al.The functional diversity of protein Iysine methylation[J].Molecular Systems Biology,10(4):724-749.
[13] Farmer S R.Brown fat and skeletal muscle:Unlikely cousins[J].Cell,2008,134(5):726-727.

[1]	REN Hao, ZHU Yixuan, CHAO Tingting, WANG Xiaoyi, LU Shaoxiong, YANG Yongli, CHEN Qiang, LI Mingli. Identification and Functional Prediction of lncRNA in Longissimus Dorsi Muscle of Saba Pigs with Different Growth Rates [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2494-2505.
[2]	DU Jiawen, REN Wenyi, XU Xiaofeng, ZHANG Lili. Comparison of the Transcriptome of Longissimus Dorsi Muscle of Tan Sheep at Different Growth Stages [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1011-1021.
[3]	WANG Yong, NA Risu, LIANG Jianyong, LIU Yang, LI Jiuyue, GAO Yuan, TIAN Feng, XUE Shuyuan. Effects of Quercetin on Follicular Development,Follicular Fluid Microenvironment and Ovarian Steroidogenesis Related Gene Expression in Mongolian Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1150-1158.
[4]	SHEN Yangyang, AN Zhenjiang, GAO Linna, XIA Shuwen, CHEN Kunlin, DING Qiang, GAO Yundong, XUE Jinguo, ZHONG Jifeng, WANG Huili. Comparative Analysis of Muscle Transcriptome of Xuzhou Cattle with Different Tenderness [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 522-533.
[5]	HUANG Yongpeng, RAN Xueqin, NIU Xi, LI Sheng, HUANG Shihui, WANG Jiafu. Gene Expression Change and Its Correlation Analysis with Meat Quality During Postmortem Pork Aging [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1573-1581.
[6]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(6): 2380-2387.
[7]	DU Jiawen, REN Wenyi, KANG Yan, XU Xiaofeng, ZHANG Lili. Transcriptome Comparison and Fatty Acid Analysis of Muscle Tissue in Tan Sheep at Different Ages [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(12): 4925-4934.
[8]	ZHU Cui, HUANG Kaiyong, LI Lina, WU Aiji, WU Peng, FU Fangmiao, LUO Yuanhua, ZHANG Huihua. Effects of Berberine on Organ Index, Antioxidant Capacity and Intestinal Immunity in Yellow-feathered Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(3): 932-941.
[9]	SHI Zhuoyan, LIU Xiaoping, JI Xiang, CHU Xiaoran, HOU Junjie, SONG Zhen, LI Xiaoxia, YANG Youbing, ZHANG Guluan, WANG Jianping, WEN Fengyun. Developmental Changes of Muscle Fiber Histology and Myogenic Regulatory Genes with Different Slaughter Weights in Yuxi Black Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(12): 4582-4592.
[10]	ZHANG Junxia, HE Na, LI Mingming, LYU Cailing, CUI Yajie, LANG Xia, WANG Cailian. Cloning,Bioinformatics and Tissue Expression Analysis of MSTN Gene in Tibetan Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(11): 4307-4317.
[11]	ZHENG Haiying, YANG Chunyan, LI Lingyu, TANG Liping, ZHENG Wei, SHANG Jianghua. Effects of Resveratrol on Proliferation of Cumulus Cells Cultured in vitro and mRNA Expression Levels of Related Genes in Buffalo [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(1): 208-215.
[12]	DING Hao, LIN Yueyue, ZHANG Tao, ZHANG Shanshan, WU Yulin, DUAN Yanjun, GONG Yongshuang, XIE Kaizhou, WANG Jinyu, DAI Guojun, ZHANG Genxi. Study on the Expression of m6A Methylation in Chicken Muscle Growth and Development [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(5): 1525-1534.
[13]	LI Qianwen, YANG Zhen, YANG Yanan, WANG Zhengwen, WEN Yuliang, CAI Yuan, ZHAO Shengguo. Effects of Environmental Temperature in Winter and Summer on Physiological Characteristics and Related Gene Expression of Tibetan Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(3): 873-881.
[14]	TANG Na, JIANG Juncai, WANG Mengdi, CHA Xuedian, ZHANG Yongyun, YANG Zhong, LI Weizhen. Expression of Genes Related to Lipid Droplet Formation in Mammary Tissue of Dehong Dairy Buffalo with Different Milk Fat Contents [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(12): 4545-4553.
[15]	WU Junzhe, XU Xueyu, WU De, CHE Lianqiang, FANG Zhengfeng, FENG Bin, XU Shengyu, LI Jian, ZHUO Yong, LIN Yan, TAO Zhiyong. Effect of Different Energy Levels of Pregnant Sow on the Testicular Development and Immunity of Male Offspring [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(11): 3962-3974.

Cloning,Bioinformatics and Tissue Differential Expression Analysis of PRDM16 Gene in Qinghai Plateau Yak

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments