低氧分压通过低氧诱导因子(HIF)影响卵母细胞的体外成熟和早期胚胎的发育

doi:10.16431/j.cnki.1671-7236.2015.06.035

Abstract

Abstract: Oocytes and early embryo in vitro culture (IVC) was a key technology of mammalian embryo engineering and was the basis of biotechnology such as genetically modified (GM) and cloning research.The factors,which affect the efficiency of in vitro culture for oocytes and early embryo,include the composition,ion concentration and osmotic pressure of the culture medium,and the gas composition and temperature of the culture environment. Recent studies have shown that the oxygen tension in the culture systems can significantly affect the development of embryos,and this effect is mainly regulated by the hypoxia-inducible factor (HIF). This paper reviewed the effects of the oxygen tension on oocyte maturation and early embryo development,and the structure and regulation mechanism of HIF were discussed,in order to provide a theoretical basis for further explore the application of hypoxia method in the in vitro culture of oocytes and early embryo.

Key words: HIF; oxygen tension; oocyte; early embryo

CLC Number:

S814.6

LIU Xiao-lin, DU Feng-jiao, LEI Chuan, RUAN Qiu-yan, WU Zhu-lian, SUN Hong-liang, ZHAO Xin, DENG Yan-fei, LU Feng-hua, SHI De-shun. Effects of Low Oxygen Tension for in vitro Oocyte Maturation and Early Embryo Development through Hypoxia-inducible Factor (HIF)[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(6): 1559-1565.

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Effects of Low Oxygen Tension for in vitro Oocyte Maturation and Early Embryo Development through Hypoxia-inducible Factor (HIF)

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