Abstract: The assay was aimed to explore the feeding and the best method to establish allergic rhinitis (AR) model in Hartley guinea pigs.Using the single factor experiment, the assay studied the effects of animal species, feeding way, ovalbumin (OVA) sensitization dose, time for AR model, and observed allergic reaction symptoms and nasal mucosal pathological section of Hartley guinea pig.The results showed that Hartley guinea pig was the best experimental animal for the AR experiment of nasal drops;Feed was ordinary mice feed + lettuce leaves or leaf lettuce;OVA as the sensitizer, concentration of basic sensitization was 0.1 to 0.3 mg/mL OVA, the next day intraperitoneal injection 1 time, for 7 times;Concentration of stimulated sensitization was 2% OVA, 10 to 30 μL each nostril, 1 time a day, after 6 to 9 d, compared with the blank group, the Hartley guinea pig model group had significant allergic symptoms and nasal mucosa lesions.This modeling method was simple and economic, good reproducibility, and could be used as the best method for AR model establishment of nasal drops.

Key words: Hartley guinea pig; feeding; nasal drops; allergic rhinitis (AR); modeling method