绵羊感染伪狂犬病的病理学观察与病毒分离鉴定

doi:10.16431/j.cnki.1671-7236.2015.02.020

Abstract

Abstract: To diagnose a suspected sheep pseudorabies case,the tissue samples of sheep with clinical symptom of sheep pseudorabies from a farm in Shanghai suburb were collected for pathological observation,isolation and identification of the virus.The histopathological observation of sheep showed extensive neuronal degeneration and necrosis occurred in the brain tissue accompanied with neurotropic phenomenon and gliosis.BHK-21 cells inoculated by tissue samples showed lesions.Pseudorabies virus (PRV) in BHK-21 cells was confirmed by specific fluorescence of indirect immunofluorescence assay and fluorescence PCR.Epidemiological investigations showed sheep pseudorabies was possibly caused by swine pseudorabies virus.Sheep pseudorabies was controlled in time by inoculating swine pseudorabies attenuated vaccine.

Key words: sheep pseudorabies; histopathology; cell culture; fluorescence PCR

CLC Number:

S855.3

QI Xin-yong, LIU Jian, ZHOU Jin-ping, JU Hou-bin, GE Fei-fei, ZHANG Wei-yi, XU Feng, SHEN Li-ping, NING Kun, WANG Jian, LIU Pei-hong. Pathological Observation of Sheep Infected Pseudorabies Virus, and Isolation and Identification of the Virus[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(2): 365-369.

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Pathological Observation of Sheep Infected Pseudorabies Virus, and Isolation and Identification of the Virus

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