猪繁殖与呼吸综合征病毒糖基化囊膜蛋白5原核表达载体的构建及免疫原性分析

Abstract

Abstract: In order to analyze the immunogenicity of glycosylated envelope protein 5 (GP5) from porcine reproductive and respiratory syndrome virus (PRRSV), ORF5 gene fragment was amplified by RT-PCR from the PRRSV (GenBank: HQ701732.1). Based on the ORF5 gene sequence, two pairs of primers were used to amplify two gene fragments, excluding the signal peptide sequence and transmembrane regions. The two gene fragments were cloned into the prokaryotic expression vector pET-28a（+）, and then the recombinant plasmid was transformed into Escherichia coli. The results of Western blotting and ELISA respectively showed that the expression of GP5 could be recognized by positive serum antibody of PRRSV, and corresponding antibodies of GP5 protein could produce in the immunized BALB/c mice. Therefore, the recombinant GP5 protein had good biological activity, and could provide fundamental data for the further study on the structure and function of GP5 protein of PRRSV.

Key words: porcine reproductive and respiratory syndrome virus; glycosylated envelope protein 5; recombinant protein; ELISA; Western blotting

LI Xin-xian, ZHANG Xiao-dan1, LIU Jing-jing, MIAO Zeng-min2, ZHANG Tao-tao, CHAI Tong-jie. Prokaryotic Expression Vector Construction and Immunogenicity Analysis of Glycosylated Envelope Protein 5 from Porcine Reproductive and Respiratory Syndrome Virus[J]. China Animal Husbandry & Veterinary Medicine.

References

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Prokaryotic Expression Vector Construction and Immunogenicity Analysis of Glycosylated Envelope Protein 5 from Porcine Reproductive and Respiratory Syndrome Virus

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