China Animal Husbandry & Veterinary Medicine ›› 2014, Vol. 41 ›› Issue (11): 40-43.

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Cloning and Sequence Analysis of GP5 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains

WU Xiao-wei1, LIU Xiao-hui2, YANG Qian3, XIONG Rui2, GUO Feng-liu2, GUO Xiao-feng3, YANG You-tian3, CHEN Ru1   

  1. 1. Guangdong Entry-exit Inspection and Quarantine Bureau, Guangzhou 510642, China;
    2. Baoding Entry-exit Inspection and Quarantine Bureau, Baoding 071051, China;
    3. South China Agricultural University, Guangzhou 510640, China
  • Received:2014-04-04 Online:2014-11-20 Published:2014-12-06

Abstract: In order to reveal the Guangdong region of porcine reproductive and respiratory syndrome (PRRS) epidemic law, 5 strains of swine clinical samples amplified from Guangdong region of PRRSV GP5 genes of the isolates with the method of RT-PCR, cloned, sequenced and domestic and foreign strains corresponding gene nucleotide sequence comparison in this study.The results showed that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains with the American type reference strains (ATCC VR-2332 U87392) nucleotide sequence homology were 88.8%, 99.5%, 88.7%, 88.7% and 83.5%,respectively. The phylogenetic tree analysis indicated that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains belonged to the American.The results provided the basis for a better understanding of the molecular epidemiology of PRRSV features and antigenic variation law, also laid the foundation for the study of gene engineering vaccine.

Key words: porcine reproductive and respiratory syndrome; GP5 protein; sequence analysis

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