猪圆环病毒2型广东分离株ORF2基因的克隆和序列分析

Abstract

Abstract: In order to clarify genotype and genetic variation of porcine circovirus type 2 (PCV2) prevalent in some regions of Guangdong province,one pair of specific primers was designed according to the published ORF2 gene sequence of PCV2,and ORF2 genes of PCV2 strain BL,HD,Li,GD01,GD02,HD01,HD02 and HD03 isolated from Guangdong province were amplified. The PCR products were cloned into pMD18-T vector and the recombinant plasmids were obtained and sequenced. The results indicated that the ORF2 gene of PCV2 Guangdong isolates had homologies ranging from 89.7% to 100% at nucleotides level and 87.2% to 99.6% at amino acids level with other PCV2 isolates in GenBank. Phylogenetic analyses revealed that 7 stains of isolates were clustered into genetic subgroup PCV2b-1A/1B,isolate HD01 was clustered into genetic subgroup PCV2b-1C.

Key words: porcine circovirus type 2; ORF2 gene; sequence analysis

CLC Number:

S852.5

SHEN Hai-yan, LIU Zhi-cheng, GUO Hui-xia, ZHOU Heng, ZHU Yu-jun, ZHANG Chun-hong. Cloning and Sequence Analysis of ORF2 Gene of Porcine Circovirus Type 2 Isolated from Guangdong Province[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(10): 12-16.

References

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Cloning and Sequence Analysis of ORF2 Gene of Porcine Circovirus Type 2 Isolated from Guangdong Province

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