Abstract: sM, M, S gene of PEDV vaccine stain were implified by amplified by RT-PCR and reorganization PCR, then inserted into the pShuttle-CMV vector. These recombinant shuttle plasmids were linearized by PmeⅠand then transformed to BJ5183 cells containing the pAdEasy-1 bone plasmid. The homologous recombinantplasmidwas transformed to XL-10 Gold ultracompetent cells in order to obtain this plasmid in a large scale. These recombinant adenoviruses were co-infected Vero cells, mice immune experiment indicated that antibodies were induced by Western blotting.

Key words: PEDV; recombinant adenovirus; vaccine; immunity