Abstract: The biocharacteristics of Nsp10 of HP-PRRSV were analyzed which in basis to express it and establish the ELISA method. BioEdit,DNAStar,PsortⅡ,SignalP,TMHMM,ProtFun,Clustal_1.81,Mega software packages were used to predict the physical and chemical properties,hydrophilicity,signal peptide,transmembrane,surface probability plot,antigenic index,secondary structure,subcellular localization and homology within the amino acid sequences of Nsp10. The predicted molecular weight was 26.38 ku and pI was 8.93. It was a stable protein and had no signal peptide and transmembrane. The study showed that Nsp10 possessed potential antigenicity and it mainly exerted the biological effects in the cytoplasm. The results showed that Nsp10 protein had highly homology with other PRRSV strain which selected. Nsp10 protein was a soluble protein with good conservatism and antigenicity. It can be a target protein used as coating antigen for ELISA detection.

Key words: highly pathogenic PRRSV JL strain; Nsp10 gene; bioinformatics prediction