China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (9): 30-33.

• 生理生化 • Previous Articles     Next Articles

Isolation and Identification and Probiotic Characteristics Study of a New Feeding Lactobacillus

SUN Xiao-wen1, LIU Qing2, YAN Yi-jie1, WANG An-ru1, WANG Pan1   

  1. 1. State Key Laboratory of Direct-Fed Microbial Engineering, Da Bei Nong Group, Beijing 100193, China;2. State Key Laboratoriy of Agrobiotechnology, China Agricultural University, Beijing 100193, China
  • Received:2010-12-14 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

Abstract: A new Lactobacillus was isolated from a faecal sample of pig. The 16S rDNA gene was sequenced, and it was submitted to GenBank, the accession number was HQ641209.Online BLAST with other Lactobacillus and phylogenetic analysis based on the fragment of 16S rDNA reveal that its sequence homology was 97.9% comparing with Lactobacillus johnsonii strain (AB295648). The isolated strain was identified as Lactobacillus johnsonii and named Lactobacillus johnsonii Sun001. L. johnsonii had high level tolerance to the simulated gastric juice (pH 2.5, 3 h of incubation, survival rate was 68.8%) and simulated gastric juice (bile salt concentration 3%,3 h of incubation, survival rate was 21.4%) and high antibacterial activity to E. coli K88, K99.

Key words: Lactobacillus johnsonii; probiotic; isolation and identificaton

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