Abstract: We studied the potential toxicity of citric acid, calcium and combination of them on mice by determining activity of serum of aspartate aminotransferase and alanine aminotransferase. Kunming mice with body weight of (20±2)g were randomly divided into control group, low, middle, high citric acid dose group, low, middle, high calcium dose group, low, medium and high citric acid joint calcium dose group. Every mouse was injected 0.2 mL corresponding reagents by intraperitoneal injection twice. When the seventh day after the second injection, the mice were picked eyeball for blood and determine activity of GOT and GPT in serum. The results showed that compared with the control group, the activity of GPT of high-dose calcium group increased 51.66%, which was significantly different (P<0.05);the activity of GPT of middle-dose calcium group and combined high dose group were significantly increased 42.63% (P<0.05), 35.32% (P<0.05) respectively .Compared with the control group, the activity of GOT in every group had no significantly difference. This research showed that the dose of Ca²⁺ can cause damage of liver cell and after combined with citric acid, liver toxicity was reduced.

Key words: citric acid; calcium; toxicity; GOT; GPT