Abstract: The present experiment was designed to feed 5 Kazak male sheep with gossypol of 0, 984, 1353 and 1722 mg/d respectively during the four periods, each period last 18 d, total 72 d. The results showed that water consumption, urine output, wheat straw feed intake, faecal output and the moisture content of feces of sheep were not significant change(P>0.05) with the amount of gossypol increasing;the level of antidiuretic hormone, rennin and cortisone didn’t change significantly, the level of adreno cortieotropic hormone and aldosterone were both decline at the previous three periods, but increased during the fourth period compare to the third period(P<0.05);The level of Cl^- and Ca²⁺ in serum decreased, and the level of Cl^- of the third period was extremely significant lower than the first period(P<0.01).As for the Ca²⁺, the third period was significant lower than the first period(P<0.05),the level of K⁺,inorg-P,Mg²⁺ in serum didn’t change significantly(P>0.05);the content of Na⁺ and inorg-P didn’t change significantly(P>0.05), the content of Cl^- was increased at the previous three periods,but the forth period return to the level of the first period,while the content of K⁺,Mg²⁺,Ca²⁺ of the forth period were lower than the previous three periods(P<0.05).

Key words: gossypol; Kazak sheep; water consumption; hormone