Abstract: Developed a method of Sodium periodate to synthesis artificial antigen SMZ-BSA and SNZ-OVA. The products were successful though UV scanning and SDS-PAGE. Choosing SMZ-OVA as coating antigen to construct indirect competition ELISA. Sulfamethazine was spiked to egg and chicken and was detected by the ELISA and HPLC. The results showed that its detection limit was 0.18 ng/mL with an assay range from 0.1 to 50 ng/mL. The LOD was 2.0 and 5.0 ng/g when sulfamethazine was spiked into egg and chicken. Compared to HPLC,the research had a high consistency with it,and could use to screen of a large number of samples for sulfamethazine residue in egg and chicken.

Key words: Sulfamethazine residue; ELISA; synthesis antigen; egg; chicken