China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (5): 78-81.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequencing Analysis of FSHβ and FSHR Gene between Wild and Domestic Wusuli Raccoon Dog

YANG Jian1, BAI Xiu-juan1,2   

  1. 1. College of Life Science, Jilin Agricultural University, Changchun 130118, China;2. College of Traditional Chinese Medicinal, Jilin Agricultural University, Changchun 130118, China
  • Received:2010-11-15 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

Abstract: In wild and domestic Wusuli raccoon dog, the FSHβ gene partial sequence, FSHR gene 5' upstream regulatory region and exon 10 were amplified and sequenced. The length of these sequences were 1257, 757 and 1398 bp, respectively, the GenBank accession numbers were HQ385977, HQ385978 and HQ385979. The homology were 99%, 98% and 98% for the exon sequences of these three genes with dog by BLAST. By multiple alignment analysis of these three genes with DNAMAN software, 12, 10 and 3 variable sites were detected, there were 2, 1 and 0 mutation sites occurred in the coding region. 6, 9 and 2 polymorphic sites of these three genes were detected comparing two wild raccoon dogs, respectively. While only 2, 1 and 0 polymorphic sites occurred for two domestic raccoon dogs. The results demonstrated that wild raccoon showed more genetic diversity.

Key words: Wusuli raccoon dog; FSHβ gene; FSHR gene

CLC Number: