Abstract: To isolate porcine reproductive and respiratory syndrome virus (PRRSV), the morbid materials were collected from some swine farm of Liaoning province and inoculated on Marc-145 cell after disposal. After blind transfer of culture, two portions showed typical cytopathic effect (CPE). TCID₅₀ of virus was 10^-6.5/mL. By the indirect immunofluorescence experiment,the yellow green fluorescence occurred in the cell cytoblastema. The virus appeared typical porcine reproductive and respiratory syndrome virus form: diameter was about 50-70 nm,circle with peplos and peplomer. It was inactivated by chloroform, ultra-violet,pH variation, and heat. The cell cytoblastema which showed CPE were tested by RT-PCR showed positive. The laboratory animals appeared obvious clinical symptom and death after inoculation. The result showed that PRRSV was identified from Liaoning province.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); isolation; identification