Abstract: Taking Tan sheep ear margin as the study material, we established successfully a fibroblast cell line by the method of explant culture directly. Observations on morphology, dynamic growth, determination of viability, analysis of karyotype, test of microorganism, transfecting fibroblasts with fluorescent protein vectors mediated(PEGFP-N3) by lipfectamine and another characteristics were detected. The results showed that population doubling time (PDT) of cells was approximately 36 h, cell viability was 95.8% after thawing, the status of cell After passage was constant, analysis of chromosomal karyotype indicated that diploid (2n=54) account for 97.5% in the cell line. Every index in the cell line met all the standard quality controls of ATCC。

Key words: Tan sheep; fibroblast cell line; gene transfection; biological characteristics