China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (5): 166-169.

• 疾病防治 • Previous Articles     Next Articles

The Isolation and Identifcation of Actinobacillus Pleuropneumoniae and Cloning and Sequence Analysis of ApxIVA Specific Fragment in Xinjiang

HAO Cheng-wu, WANG Yong-xia, LI Shang-hua, MA Xun   

  1. (College of Animal Science and Technology, Xinjiang Shihezi University, Shihezi 832003, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: MA Xun

Abstract: A strain of Actinobacillus pleuropneumoniae（APP） is isolated from 20 cases of swine which may be infected with APP from 4 large-scale pig farms in the north of Xinjiang. The strains were identified by morphologic, cultural and biochemical characteristics. And according to the sequence of APP, the 2427 bp of N-terminal of ApxⅣA was amplified by PCR cloned into pMD19-T vector and sequence analyzed. The results of DNA sequence comparison of the amplified fragment with the published sequence in GenBank showed 98% nucleotide homology, comparing with the three standard strains. The homlogy is separately 98.23%, 99.46%, 98.06%.

Key words: isolation; identification; ApxⅣ cloning; sequencing

CLC Number: