Abstract: To study effect of culture time and epidermal growth factor on the in vitro maturation of rabbit oocytes. After superovulating female rabbit, we killed it and took out it’s ovarians, washed them and obtained COCs on the surface of ovarian by method of cutting with blade or by stab-extruding with niddle, observed the emission rate of the first polar body at different times (24, 30, 36 and 40 hours) and added in the basic culture medium with different concentrations (0, 50, 100 ng/mL) of epidermal growth factor (EGF) in vitro maturation. The results showed that： ①Method of stab-extruding with niddle to obtain the number of COCs was bitter than cutting with blade (P<0.05); ②the first polar body emission rate at in vitro maturation time of 30 (36) hours of was higher than 24 (40) hours (P<0.05); ③the first polar body emission rate in adding 50 ng/mL and 100 ng / mL EGF group was significantly higher (P<0.05). Therefore,
①Method of stab-extruding with niddle was better than method of cutting with blade in obtaining COCs on the surface of the rabbit ovarian；②On in vitro maturation of rabbit oocyte, time of 30 to 36 hours was appropriate；③EGF could significantly improve oocytes maturation, it was suitable to add 100 ng/mL EGF in the basic culture medium.

Key words: rabbit; oocyte; in vitro maturation; EGF