Abstract: 30 Kunming mice were divided into 3 groups random to establish the mouse mastitis patho-model. 30 mice were injected separately with 50 μL sterile physiological saline，1.0×10⁵ CFU/mL and 2.0×10⁵ CFU/mL Staphylococcus aureus in the forth lacteal gland. The appearance of lacteal gland and the diversity of histological section were observed. The results indicated that the mice 24 h after being injected with bacterium appeared evident clinical symptoms; the different degree inflammation was found in the lacteal gland; plenty of neutrophilic granulocyte infiltrated in the acinar lumina and the compartment became widen were discovered by the method of histopathology. The result indicated that the mouse mastitis model was established by the Staphylococcus aureus.

Key words: mouse; Staphylococcus aureus; mastitis; patho-model