鉴别布鲁菌病自然感染动物与疫苗免疫动物胶体金检测试纸条的研制

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (7): 79-82.

• 生物技术 • Previous Articles Next Articles

Development of Colloidal Gold Test Strip-discern Infected and Vaccinated Animals by Brucella

ZHANG Fu-xian^1,2, LI Xiao-yan², YAN Guang-mou¹, TANG Jie², YANG Yan-ling¹, LU Hao¹, LANG Xu-long², WANG Xing-long²

（1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2.The Military Veterinary Institute Academy of Military Medical Sciences of PLA, Changchun 130062, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
Contact: WANG Xing-long

Abstract

Abstract: SPA labeling technique for the gold-labled pond, BP26 purified by affinity chromatography for the detection antigen(T line), the colloidal gold test strip was designed to detect Brucellosis antibody, to discern infected mouse by Brucella abortus S19 and vaccinated mouse by BP26 KO Brucella strain YZ-2. There was no cross-reaction between the Brucella and the bacteria which have highly homology to Brucella for the strip test. Compared to other general tests, the sensitivity of the strip test is more than RBPT, and similar to ELISA; the coincidence of test results with ELISA was 100%, and the strip test was obviously more convenient. The colloidal gold test strip have the potential application to discern infected and vaccinated animals by Brucella.

Key words: Brucellosis; BP26 protein; colloidal gold test strip; discern

CLC Number:

S855.1⁺2

ZHANG Fu-xian;LI Xiao-yan;YAN Guang-mou;TANG Jie;YANG Yan-ling;LU Hao;LANG Xu-long;WANG Xing-long. Development of Colloidal Gold Test Strip-discern Infected and Vaccinated Animals by Brucella[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(7): 79-82.

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Development of Colloidal Gold Test Strip-discern Infected and Vaccinated Animals by Brucella

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