China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (7): 75-78.

• 生物技术 • Previous Articles     Next Articles

T-RFLP Analysis Technique and its Application on Analyzing Microbial Community Structure

LUO Jia-jie1,2, LI Li-li1, ZHANG Bin2   

  1. （1.The Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125，China；2.Institute of Economy Animal Sciences，Changsha 410128，China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: LI Li-li

Abstract: The advance of terminal restriction fragment length polymorphism(T-RFLP) in analyzing microbial community structure in environment,animal and human as well as the fundamental principle and operational approach of T-RFLP were detailed. T-RFLP has many advantages over the other genetic fingerprinting profiles on analyzing microbial community structure owning to its higher resolving power and higher through output by integrated with automatic sequencers and its culture-independent. In addition, the disadvantages and the foreground of this technique were summarized.

Key words: T-RFLP; microbial community structure; fingerprinting; automatic sequencing

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