Abstract: A pair of primers was synthesized according to the sequence of PRV Ka strain. The fragment containing gK gene coding domains was amplified by polymerase chain reaction (PCR) using BamH I 5′ fragment of PRV Ea strain as template. The fragment was cloned into pBluescriptIISK+, resulting recombination plasmid pSKgK. Then the gK gene was cloned into eukaryotic expression vector pEGFP-C1, following the EGFP (enhanced green fluorescent protein). The recombinant plasmid pEGFPgK were transfected into BHK-21 cell with lipofectin and selected under G418, until the normal BHK-21 cell die out. Expression protein was detected under inverted fluorescence microscope. The results showed that gK gene was expressed in the cytomembrane and cytoplasm of BHK-21 cell.

Key words: PRV; gK gene; eukaryotic expression