3种提取基因组DNA方法的比较分析

Abstract

Abstract: We separately used several different method to extracted genomic DNA,
compared with several methods as well as the time, the expense and the time to choose an efficient, simple fast, reasonably priced extraction methods. Finally: Several methods could achieve the quality of molecular biology experiments related requirements, but in the sequence of operation, the time and the price there exist differences.Specific results of this comparison to be possible to provide the basis for the molecular biology experiment selective extraction method.

Key words: DNA; extraction; blood; organizations; FTA sampling card

CLC Number:

Q503

LIU Yan;LIU Meng-zhou;XU Yan-xiang. Comparison of Three Methods for Genomic DNA Extraction[J]. China Animal Husbandry & Veterinary Medicine, 2008, 1(4): 45-47.

[1]	ZHAO Jinghong, MA Hui, LIU Jintao, MIAO Renfang, LIU Hongzhi, LIU Duncheng, QU Yongli. Effects of Supplemental Light on Blood Endocrine Hormone Concentrations and Circadian Rhythm Changes in Late-gestation Holstein Breeding Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3178-3189.
[2]	XU Baichang, XIE Zonggu, SI Hongbin. Research Progress on Extraction Techniques,Structural Characterization and Biological Activities of Polysaccharides Derived from Broussonetia papyrifera [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2662-2674.
[3]	LIU Dandan, LUO jie, GAO Hanya, XU Liyina, HAO Baoshan, WANG Xin, ZHANG Wei. Optimization of the Extraction Process of Crude Polysaccharides from Nymphaea candida and Its Therapeutic Effect on Mouse Mastitis Induced by Staphylococcus aureus [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2818-2829.
[4]	LIU Min, CHENG Huai, ZHENG Zhenzhen, ZHANG Mengsi, ZHANG Hewei, REN Jingqiang. Eukaryotic Expression and Polyclonal Antibody Preparation of N Protein of Canine Parainfluenza Virus [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2287-2294.
[5]	LIANG Jiahao, SHI Yuangang, KANG Xiaolong. Research Pogress on Histone Acetylation Modification and Its Role in the Regulation of DNA Damage Repair [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1499-1510.
[6]	ZHANG Zhengfei, TANG Anxing, SUN Jindong, FU Lixiang, WANG Xingxian, ZHANG Shiyun, YANG Liangyu, NIU Guoyi, TAO Linli. Study on the Appropriate Dietary Crude Protein Level of Yanjin Black-bone Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1543-1553.
[7]	ZHOU Xin, YANG Cheng, ZHU Guiming, HE Qianrong, LI Yan, FAN Xinzhong, NING Chao, WANG Dan. NUMTs Mapping of Rabbit Genome Based on the Latest Reference Sequence UM_NZW_1.0 [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1660-1670.
[8]	WANG Panpan, SHARAMATI·Bodai, BAKHET·Bodai, LI Zhenwei, ORALHAZI·Hazikhan. Analysis of Genetic Structure and Maternal Origin of Seven Kazakh Cattle Populations in Xinjiang Based on D-loop Polymorphism [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1166-1179.
[9]	MAN Jingyuan, LIU Yu, XING Xiaoyong, YANG Zhen, HAO Baocheng, LABA Cidan, HE Junfeng, WUJIN Cuomu. Optimization of Extraction Process of Polysaccharides from Polygonum viviparum L.by Response Surface Methodology [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1416-1427.
[10]	ZHONG Ming, GENG Longjun, CUI Lianhua, XU Ziyan, YAN Changguo, FENG Jian, CAI Hongfan, ZHANG Xu. Effects of Curcumin on Growth Performance, Apparent Digestibility of Feed Nutrients and Blood Indexes of Yanbian Yellow Breeding Cattle Under Heat Stress [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 655-667.
[11]	DING Mengjie, WANG Pingping, HU Longfei, ZHANG Qiuna, CHEN Jing, LIU Jia, LIU Ruonan, HAO Zhihui. Safety Test of Banqing Granules on Target Animal Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4164-4173.
[12]	YAN Gang, XING Tianqi, WANG Yubin, JIANG Shan, ZHANG Shuai, XU Di, ZHANG Kun, WANG Mengying, LIANG Jing, LAN Ganqiu, LI Kui, HUANG Sanwen. Effects of Substituting Dried Potatoes for Corn on Growth Performance,Meat Quality Traits and Routine Blood Indexes of Growing-finishing Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3345-3354.
[13]	ZHANG Jirong, ZHANG Guoqing, Bolatjan·Alipbek. The Effect of Supplementing Yeast Culture on Milk Production,Milk Composition and Blood Biochemical Indicators in Lactating Mares [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3410-3416.
[14]	HE Xingli, ZHOU Peiyao, ZHANG Xiaoxue, MOU Quanzhou, LI Yang, WANG Zhaoyuan, LIU Peng, WANG Zi, SONG Yangyang, LI Xiaolin, SHEN Binglei. Effect of Staphylococcus aureus and Staphylococcus epidermidis on the Secretion of Inflammatory Factors by Neutrophils in Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3676-3686.
[15]	REN Feier, YANG Fan, LI Minghao, LIANG Yingtao, REN Jie, ABDURAHMU Ehmeti, CHEN Kaixu. Effects of Different Levels of Dietary Ellagic Acid Supplementation on Growth Performance,Blood Biochemical Indices and Antioxidant Capacity of Kazakh Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2899-2907.

Comparison of Three Methods for Genomic DNA Extraction

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments