捻转血矛线虫虫卵的大量分离纯化

China Animal Husbandry & Veterinary Medicine ›› 2008, Vol. 1 ›› Issue (12): 120-122.

• 疾病防治 • Previous Articles Next Articles

Separation and Purification of Much Eggs from Haemonchus Contorts

RONG Guang^1,2, ZHAO Junming¹, ZHENG Jinhai¹, WANG Xinhua^1, BO Xinwen¹, ZHONG Fagang¹, LUO Panqi¹, REN Yaojun¹

1.The Key Sheepbreeding Laboratory of Xinjiang Production and Construction Group,Shihezi 832003，China; 2. Tropical Crop Genetic Resources Institute,CATAS, Danzhou 571737,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

Abstract

Abstract: By gathering eggs from female adults of H. contortus,and hatching their eggs in incubation bottles,lots of monospecifical infectious larvae on the lids of these bottles were obtained, and contanmination was avoided from other worms.It was successful with these larvae infencting sheep. Then a lot of purebred L3 stage larvaes from the haemonchus contorts eggs were collected and got by salt floatation in the faeces of monospecifically infected sheep.The method provides a way to get purebred larvae in molecular biology research of parasites.

Key words: haemonchus contorts; eggs; separation and purification

RONG Guang;ZHAO Junming;ZHENG Jinhai;WANG Xinhua;BO Xinwen;ZHONG Fagang;LUO Panqi;REN Yaojun. Separation and Purification of Much Eggs from Haemonchus Contorts[J]. China Animal Husbandry & Veterinary Medicine, 2008, 1(12): 120-122.

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Separation and Purification of Much Eggs from Haemonchus Contorts

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