Abstract: The author separates and extracts the overall DNA from leg muscles of mink, then gets the DNA sequence of mink FSH-β gene after PCR amplification. The product of PCR amplification is then conjunction onto pMD-18T carrier after gelatum reclaim, then transformed into competence cell JM109. bolting masculine clone colony on LB(Amp+) medium which contains X-gal and IPTG. Then after plasmid extraction， restriction enzyme cut and PCR accredit, the target segment is sequencing. As a result of the test, the amount of basic group of DNA segment after PCR amplification is 500bp, by contrast with Blast, the author analyzes the home ology of follicle-stimulating hormone ribonucleotide sequence between mink and human being, ox, pig, sheep, mouse.

Key words: mink; FSH-β gene; clone; sequence analysis