马乳酒样乳杆菌JK-24对DSS诱导的小鼠结肠炎的缓解作用

doi:10.16431/j.cnki.1671-7236.2025.09.036

Abstract

Abstract: 【Objective】 The purpose of this experiment was to investigate the alleviation effect of Lactobacillus kefiranofaciens JK-24 on dextran sodium sulfate (DSS)-induced colitis in mice. 【Method】 18 C57BL/6 mice were randomly divided into 3 groups:Blank control (Control),model (DSS) and Lactobacillus kefiranofaciens JK-24 (JK-24＋DSS) groups，and 6 mice in each group.From the first day of the experiment,mice in JK-24＋DSS group were gavaged with Lactobacillus liquidacidum JK-24 suspension at 0.1 mL/10 g (with a concentration of 10⁹ CFU/mL).Mice in Control and DSS groups were gavaged with the same amount of normal saline for 21 consecutive days.From the 15th to the 21st day,mice in DSS and JK-24＋DSS groups freely drank 3% DSS solution,while mice in Control group drank water normally.During the experiment,the weight changes of mice were recorded,the fecal conditions were observed,and the disease activity index (DAI) was calculated.After the experiment,colon and blood samples of mice were collected.The length of the colon was measured and the histopathological changes of the colon tissue were observed.The levels of oxidative stress indicators and inflammatory cytokines in serum and colon tissue were determined. 【Result】 Compared with Control group,the weight of mice in DSS group decreased significantly,and the feces did not form or even showed bloody stools,DAI score increased significantly (P＜0.05).A large number of inflammatory cells were seen in colon tissues,the intestinal mucosal structure was destroyed.The contents of inflammatory factors interleukin-12 (IL-12),tumor necrosis factor-α (TNF-α),IL-10 and IL-6 in serum and colonic tissues were significantly or extremely significantly increased (P＜0.05 or P＜0.01).The activities of superoxide dismutase (SOD) and catalase (CAT) decreased extremely significantly (P＜ 0.01),while the activity of myeloperoxidase (MPO) and the content of malondialdehyde (MDA) increased extremely significantly (P＜0.01).Compared with DSS group,the body weight of mice in JK-24＋DSS group decreased relatively slowly,and the DAI score decreased in the later stage of modeling.The length of colon increased significantly (P＜0.05),the area of inflammatory cell infiltration in colonic tissue decreased significantly,and the structure of colonic mucosal tissue was basically intact.Only in some areas did glandular absence and irregular arrangement occur.The contents of IL-6,IL-10,IL-12 and TNF-α in serum and colonic tissue were significantly or extremely significantly decreased (P＜0.05 or P＜0.01).The activities of SOD and CAT in serum were extremely significantly increased (P＜0.01),the activity of MPO was significantly decreased (P＜0.05),and the content of MDA was extremely significantly decreased (P＜0.01). 【Conclusion】 Lactobacillus kefiranofaciens JK-24 had a significant alleviating effect on DSS-induced colitis in mice.The main mechanism of action was related to its improvement of the antioxidant function and reduction of the production of inflammatory factors.

Key words: Lactobacillus kefiranofaciens; dextran sodium sulfate(DSS); colitis; antioxidant effect

CLC Number:

S816.79

ZHANG Hua, XU Zhiqin, ZHOU Chuang, QIAO Yiran, HU Canran, LIN Jiaqi, XUN Jiaqi. Alleviation Effect of Lactobacillus kefiranofaciens JK-24 on DSS-induced Colitis in Mice[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4406-4415.

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Alleviation Effect of Lactobacillus kefiranofaciens JK-24 on DSS-induced Colitis in Mice

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