CRISPR-Cas9基因编辑技术在牛、羊生产中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2024.11.023

Abstract

Abstract: In recent years,gene editing technology has developed rapidly as a genome modification tool.Gene editing tools including zinc-finger nuclease (ZFNs),transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats associated protein (CRISPR-Cas) system make it possible to target genome DNA modification of organisms.The emergence of the CRISPR-Cas9 system,in particular,accelerated the development of gene editing technology as a revolutionary tool in basic and applied science.Compared with ZFNs and TALENs technologies,CRISPR-Cas9 systems are widely used due to their high flexibility,sensitivity,specificity and cost effectiveness.CRISPR-Cas9 gene editing technology has made significant contributions to different aspects of livestock production by precisely cutting DNA using targeting specific sequences and introducing site-specific modifications such as adding,removing or replacing nucleotides by generating double-strand breaks at specific genomic sites,producing a variety of animal models such as improved livestock reproductive production and disease resistance,so as to study the key gene functions of livestock and poultry and accelerate the improvement of traits.In this paper,the mechanism and function of CRISPR-Cas9 system and its application in the production of cattle and sheep are reviewed,in order to provide reference for future research.

Key words: CRISPR-Cas9 system; gene editing; cattle; sheep; reproduction; lactation; disease resistance

CLC Number:

S813.3

PAN Dongxia, WANG Hui, XIONG Benhai, TANG Xiangfang. Research Progress on CRISPR-Cas9 Gene Editing Technology in Cattle and Sheep Production[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(11): 4880-4889.

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Research Progress on CRISPR-Cas9 Gene Editing Technology in Cattle and Sheep Production

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