Abstract: Single-cell sequencing is a high-throughput sequencing technique based on obtaining all genetic information at the level of a single cell.It reveals the intracellular dynamics of individual cells and answer biological questions with high-dimensional catalogs of millions of cells,including genomics,transcriptomics,chromatin accessibility,epigenomics,and proteomics data across species.Within the domain of animal reproduction,single-cell sequencing has emerged as a crucial scientific tool with its powerful ability to decipher cellular and molecular landscapes at single-cell resolution.It is of great significance in solving scientific problems such as the cell classification,intercellular interactions,intercellular communication connections,and the mining of key reproductive genes in animal reproduction.This invaluable technique greatly contributes to advancing our understanding of reproductive mechanisms and enhancing animal reproductive performance.The authors briefly introduce the principles of single-cell sequencing and three types of sequencing,and summarize its role in animal reproductive physiology research,aiming to provide theoretical basis for future research endeavors.

Key words: single-cell sequencing; animal reproductive; cell classification; intercellular communication; gene mining