东方欧鳊寄生指环虫种类鉴定及系统发育分析

doi:10.16431/j.cnki.1671-7236.2023.07.027

Abstract

Abstract: 【Objective】 This study was aimed to explore the species and morphological characteristics of the Dactylogyrus parasitized in the Abramis brama orientalis in Irtysh River, and the phylogeny of Dactylogyrus parasitized in Cyprinidae, so as to provide theoretical basis for the phylogeny of Dactylogyrus and its co-evolutionary relationship with host.【Method】 From July 2021 to July 2022, morphological and molecular biological methods were used to identify and phylogenetically analyze Dactylogyrus parasitized in the gills of Abramis brama orientalis.【Result】 According to the morphological observation and chitin structure characteristics, the Dactylogyrus parasitized in the Abramis brama orientalis were identified as Dactylogyrus zandti and Dactylogyrus wunderi, and the structure of their copulatory organ was significantly different.By amplifying the 18S-ITS1-5.8S rDNA and 28S rDNA sequences of two species of Dactylogyrus, and constructing the phylogenetic tree by using Bayesian method (BI) and maximum likelihood method (ML), respectively, the Dactylogyrus zandti and Dactylogyrus wunderi were clustered into one branch, and were located in the same branch as the branchial Dactylogyrus parasitized in the subfamily of Leuciscinae.The phylogenetic trees constructed by different ribosomal sequences were basically the same.Most of the Dactylogyrus parasitized in different subfamily fishes were clustered together, and the Dactylogyrus parasitized in Cyprininae fishes were located at the base of the phylogenetic tree.In the phylogenetic tree constructed by 28S rDNA sequence, the Dactylogyrus parasitized in Cyprininae and Labeoninae fishes were clustered into one branch and located at the base of the phylogenetic tree.【Conclusion】 This experiment described the morphological characteristics of two species of Dactylogyrus parasitized in the Abramis brama orientalis, and supplemented the morphological data of Dactylogyrus.The phylogenetic trees with different ribosomal sequences indicated that there were host transfer and intra-host speciation phenomena in the co-evolution between the Dactylogyrus and its hosts.This study supported Cyprininae fishes as the early hosts of Dactylogyrus, and the phylogeny of Dactylogyrus might be influenced by host specificity and geographical location.

Key words: Abramis brama orientalis; Dactylogyrus; identification; phylogeny

CLC Number:

S852.71

WEI Nianwen, LIU Yanjun, SHI Caixia, LIU Doudou, FENG Longji, ZHANG Li, WANG Jinpu, YUE Cheng, HAO Cuilan. Species Identification and Phylogenetic Analysis of the Parasitic Dactylogyrus of Abramis brama orientalis[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(7): 2876-2887.

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Species Identification and Phylogenetic Analysis of the Parasitic Dactylogyrus of Abramis brama orientalis

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