LPS诱导马冈鹅法氏囊损伤相关基因的筛选

doi:10.16431/j.cnki.1671-7236.2023.07.005

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the effects of lipopolysaccharide (LPS) on tissue injury and oxidative stress of bursae of Fabricius of Magang geese and to explore its regulatory mechanism.【Method】 One hundred 1-day-old Magang geese (half female and half male) were randomly divided into control and LPS group with 5 replicates in each group and 10 geese in each replicate.The goslings in LPS group were injected intraperitoneally with 2 mg/kg LPS at 24, 26 and 28 days, respectively, while the control group was injected with the same amount of saline.After 1 hour of injection at 28 days, the bursa of Fabricius was collected aseptically for histological observation and oxidative stress index detection and transcriptome library construction.Candidate genes related to LPS-induced bursa of Fabricius injury were screened by differentially expressed gene identification, GO function and KEGG pathway enrichment, and protein interaction network analysis.Ten differentially expressed genes were randomly selected and the expressions were verified by Real-time quantitative PCR.【Result】 Histological observation of bursae of Fabricius of Magang goose showed that the structural integrity of bursae of Fabricius was significantly decreased, the cell arrangement in vesicles was disordered and the number of vacuoles increased in LPS group.Compared with the control group, the activity of glutathione peroxidase (GSH-Px) of bursae of Fabricius was significantly decreased in LPS group (P <0.05).A total of 507 differentially expressed genes were obtained in LPS group, of which 277 genes were up-regulated and 230 genes were down-regulated.GO function and KEGG pathway enrichment analysis showed that differentially expressed genes were mainly concentrated in items related to inflammation and immune response, and related to Cytokine-cytokine receptor interaction pathway and Notch and Hedgehog signaling pathways. A total of 27 differentially expressed genes, including TNF superfamily member 10 (TNFSF10), CREB-binding protein (CREBBP), paracentric substance 1 (PCM1), protein tyrosine phosphatase non-receptor type 6 (PTPN6), were screened by binding protein cooperative network analysis.The results of Real-time quantitative PCR showed that the transcriptome sequencing results were accurate and reliable.【Conclusion】 This study explored the differential expression genes in bursa of Fabricius in Magang goose before and after LPS-induced, and identified the Cytokine-cytokine receptor interaction, Notch and Hedgehog signaling pathways and related genes such as TNFSF10, CREBBP and PTPN6 affecting bursa of Fabricius injury, which provide theoretical reference for understanding the molecular mechanism of LPS-induced bursae of Fabricius injury in geese.

Key words: lipopolysaccharide; Magang goose; bursa of Fabricius injury; transcriptome sequencing

CLC Number:

S835

YU Ziyu, ZHANG Bingqi, CAI Yuanrong, HONG Longsheng, LI Wanyan, CAO Nan, HUANG Yunmao, XU Danning, TIAN Yunbo, CHEN Wenbin, LI Bingxin. Screening of Genes Related to Bursa of Fabricius Injury Induced by LPS in Magang Geese[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(7): 2639-2650.

References

[1] PUSSINEN P J, KOPRA E, PIETIÄINEN M, et al.Periodontitis and cardiometabolic disorders:The role of lipopolysaccharide and endotoxemia[J].Periodontology 2000, 2022, 89(1):19-40.
[2] RICHARD J F, YITZHAK T.Antibiotics and bacterial resistance in the 21st century[J].Perspectives in Medicinal Chemistry, 2014, 6:105.
[3] 施振旦, 刘丽, 孙爱东."禽-鱼"高密度养殖对水禽生产性能危害的研究[J].中国家禽, 2011, 33(13):1-3. SHI Z D, LIU L, SUN A D.Study on the harm of "Poultry-Fish" high density culture to the production performance of waterfowl[J].China Poultry, 2011, 33(13):1-3.(in Chinese)
[4] 江丹莉, 刘丽, 陈芳, 等.细菌内毒素对肉鹅生长性能及免疫机能的影响研究[J].中国家禽, 2011, 33(7):10-15. JIANG D L, LIU L, CHEN F, et al.Effects of LPS on growth performance and immune function in meat geese[J].China Poultry, 2011, 33(7):10-15.(in Chinese)
[5] BARBER M R W, ALDRIDGE J R, WEBSTER R G, et al.Association of RIG-I with innate immunity of ducks to influenza[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(13):5913-5918.
[6] EKINO S, SONODA K.New insight into the origin of IgG-bearing cells in the bursa of Fabricius[J].International Review of Cell and Molecular Biology, 2014, 312:107-131.
[7] 孙圣兰, 江清艳, 傅伟龙.法氏囊及法氏囊素的研究概况[J].畜禽业, 2002, 3:Y014-Y015. SUN S L, JIANG Q Y, FU W L.A survey of the study on bursa and bursin of Fabricius[J].Livestock and Poultry Industry, 2002, 3:Y014-Y015.(in Chinese)
[8] CALDWELL D J, DEAN C E, MCELROV A P, et al.Bursal anti-steroidogenic peptide (BASP):Modulation of mitogen-stimulated bursal-lymphocyte DNA synthesis[J].Comparative Biochemistry & Physiology, 1999, 123(4):385-391.
[9] BRAND A, GILMOUR D G, GOLDSTEIN G.Lymphocyte-differentiating hormone of bursa of Fabricius[J].Science, 1976, 193(4250):319-321.
[10] 侯艳红, 钱建飞, 郝勤宗.法氏囊活性肽的研究进展[J].中国家禽, 2001, 23(12):10-12. HOU Y H, QIAN J F, HAO Q Z, et al.Research progress of bioactive peptides in bursa of Fabricius[J].China Poultry, 2001, 23(12):10-12.(in Chinese)
[11] 于海, 陈蕾, 刘兆球, 等.法氏囊素的研究进展及应用前景[J].山东家禽, 2004, 4:48-49. YU H, CHEN L, LIU Z Q, et al.Research progress and application prospect of bursin[J].Shandong Poultry, 2004, 4:48-49.(in Chinese)
[12] 何洋, 施红梅, 杜彦丽, 等.禽法氏囊发育与免疫功能研究新进展[J].中国农业大学学报, 2022, 27(2):120-131. HE Y, SHI H M, DU Y L, et al.New Progress in the study of bursa development and immune function of poultry[J].Journal of China Agricultural University, 2022, 27(2):120-131.(in Chinese)
[13] 张冰琪, 李冰心, 杨保和, 等.白术多糖通过Toll样受体4/p38丝裂原活化蛋白激酶信号通路缓解脂多糖诱导的雏鹅法氏囊损伤[J].动物营养学报, 2022, 34(10):6737-6747. ZHANG B Q, LI B X, YANG B H, et al.Polysaccharide of Atractylodes macrocephala Koidz alleviates lipopolysaccharide induced bursa of Fabricius injury in goslings through Toll-like receptor 4/p38 mitogen activated protein kinase signaling pathway[J].Chinese Journal of Animal Nutrition, 2022, 34(10):6737-6747.(in Chinese)
[14] 李婉雁.白术多糖对雏鹅的免疫保护作用及机制研究[D].广州:华南农业大学, 2018. LI W Y.The Immunoprotective effects and mechanisms of polysaccharide of Atractylodes macrocephala Koidz on goslings[D].Guangzhou:South China Agricultural University, 2018.(in Chinese)
[15] MARTIN M.Cutadapt removes adapter sequences from high-throughput sequencing reads[J].EMBnet Journal, 2011, 17(1):10-12.
[16] SZKLARCZYK D, FRANCESCHINI A, WYDER S, et al.STRING v10:Protein-protein interaction networks, integrated over the tree of life[J].Nucleic Acids Research, 2015, 43:D447-D452.
[17] SHANNON P, MARKIEL A, OZIER O, et al.Cytoscape:A software environment for integrated models of biomolecular interaction networks[J].Genome Research, 2003, 13(11):2498-2504.
[18] XI Y, YAN J, LI M, et al.Gut microbiota dysbiosis increases the risk of visceral gout in goslings through translocation of gut-derived lipopolysaccharide[J].Poultry Science, 2019, 98(11):5361-5373.
[19] FERRECCIO C, GONZALEZ P C, MILOSAVJLEVIC S V, et al.Lung cancer and arsenic exposure in drinking water:A case-control study in northern Chile[J].Cadernos de Saude Publica, 1998, 14(Supp3):193-198.
[20] YUE Y, BING W, LINMIAO J, et al.Comparative analysis of toxicity reduction of wastewater in twelve industrial park wastewater treatment plants based on battery of toxicity assays[J].Scientific Reports, 2019, 9(1):3751.
[21] ARSLAN F, LAI R C, SMEETS M B, et al.Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury[J].Stem Cell Research, 2013, 10(3):301-312.
[22] ZHAO F Q, ZHANG Z W, QU J P, et al.Cold stress induces antioxidants and Hsps in chicken immune organs[J].Cell Stress Chaperones, 2014, 19(5):635-648.
[23] FAN Y, XIONG W, LI J, et al.Mechanism of Tangganjian on nonalcoholic fatty liver disease with type 2 diabetes mellitus[J].Pharmaceutical Biology, 2018, 56(1):567-572.
[24] CUA D J, SHERLOCK J, CHEN Y, et al.Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain[J].Nature, 2003, 421(6924):744-748.
[25] TENG M W L, BOWMAN E P, MCELWEE J J, et al.IL-12 and IL-23 cytokines:From discovery to targeted therapies for immune-mediated inflammatory diseases[J].Nature Medicine, 2015, 21(7):719-729.
[26] MAIER J, KINCAID C, PAGENSTECHER A, et al.Regulation of signal transducer and activator of transcription and suppressor of cytokine-signaling gene expression in the brain of mice with astrocyte-targeted production of interleukin-12 or experimental autoimmune encephalomyelitis[J].American Journal of Pathology, 2002, 160(1):271-288.
[27] ALBIN S, CUNNINGHAM-RUNDLES C.An update on the use of immunoglobulin for the treatment of immunodeficiency disorders[J].Immunotherapy, 2014, 6(10):1113-1126.
[28] WASIAK S, DZOBO K E, RAKAI B D, et al.BET protein inhibitor apabetalone (RVX-208) suppresses pro-inflammatory hyper-activation of monocytes from patients with cardiovascular disease and type 2 diabetes[J]. Clinical Epigenetics, 2020, 12(1):166.
[29] SUN H, CHENG Y, YAN Z, et al.Mining the proliferative diabetic retinopathy-associated genes and pathways by integrated bioinformatic analysis[J].International Ophthalmology, 2020, 40(2):269-279.
[30] ELIAS J A, ZITNIK R J.Cytokine-cytokine interactions in the context of cytokine networking[J].American Journal of Respirator Cell and Molecular Biology, 1992, 7(4):365-367.
[31] PLATA-SALAMAN C R.Brain mechanisms in cytokine-induced anorexia[J].Psychoneuroendocrinology, 1999, 24(1):25-41.
[32] TADMORI W, ZHANG M, BEAVIS A J, et al.Suppression of the allogeneic response by human IL-10:A critical role for suppression of a synergy between IL-2 and TNF-alpha[J].Cytokine, 1994, 6(5):462-471.
[33] SWIERGIEL A H, SMAGIN G N, JOHNSON L J, et al.The role of cytokines in the behavioral responses to endotoxin and Influenza virus infection in mice:Effects of acute and chronic administration of the interleukin-1-receptor antagonist (IL-1ra)[J].Brain Research, 1997, 776(1-2):96-104.
[34] STRIJBOS P J, ROTHWELL N J.Interleukin-1 beta attenuates excitatory amino acid-induced neurodegeneration in vitro:Involvement of nerve growth factor[J]. Journal of Neuroscience, 1995, 15(5):3468-3474.
[35] SHANG Y, SMITH S, HU X.Role of Notch signaling in regulating innate immunity and inflammation in health and disease[J]. Protein & Cell, 2016, 7(3):159-174.
[36] CAI Z, ZHAO B, DENG Y, et al.Notch signaling in cerebrovascular diseases (review)[J]. Molecular Medicine Reports, 2016, 14(4):2883-2898.
[37] TSAO P N, WEI S C, HUANG M T, et al.Lipopolysaccharide-induced Notch signaling activation through JNK-dependent pathway regulates inflammatory response[J]. Journal of Biomedical Science, 2011, 18:56.
[38] ERIK F, SAI M T T, JAMES P C, et al.Delta-like 4 induces Notch signaling in macrophages[J].Circulation, 2007, 115(23):2948-2956.
[39] ROWBOTHAM N J, HAGER-THEODORIDES A L, CEBECAUER M, et al.Activation of the Hedgehog signaling pathway in T-lineage cells inhibits TCR repertoire selection in the thymus and peripheral T-cell activation[J].Blood, 2007, 109(9):3757-3766.
[40] KWON H, SONG K, HAN C, et al.Inhibition of Hedgehog signaling ameliorates hepatic inflammation in mice with nonalcoholic fatty liver disease[J].Hepatology, 2016, 63(4):1155-1169.
[41] LEE J J, ROTHENBERG M E, SEELEY E S, et al.Control of inflammation by stromal Hedgehog pathway activation restrains colitis[J].Proceedings of the National Academy of Science, 2016, 113(47):E7545-E7553.
[42] YANG Y, LI Q, DENG Z, et al.Protection from lipopolysaccharide-induced pulmonary microvascular endothelial cell injury by activation of Hedgehog signaling pathway[J]. Molecular Biology Reports, 2011, 38(6):3615-3622.
[43] BURGALETTO C, MUNAFO A, DI BENEDETTO G, et al.The immune system on the TRAIL of Alzheimer's disease[J].Journal of Neuroinflammation, 2020, 17(1):298.
[44] SUN X, LIU Y, WANG J, et al.Cardioprotection of M2 macrophages-derived exosomal microRNA-24-3p/Tnfsf10 axis against myocardial injury after sepsis[J]. Molecular Immunology, 2022, 141:309-317.
[45] JIANG Y, CHEN X, FAN M, et al.TRAIL facilitates cytokine expression and macrophage migration during hypoxia/reoxygenation via ER stress-dependent NF-kappaB pathway[J].Molecular Immunology, 2017, 82:123-136.
[46] HALAAS O, VIK R, ASHKENAZI A, et al.Lipopolysaccharide induces expression of APO2 ligand/TRAIL in human monocytes and macrophages[J].Scandinavian Journal of Immunology, 2000, 51(3):244-250.
[47] ZHENG L, YANG Y D, LU G C, et al.Lipopolysaccharide (LPS) increases tumor necrosis factor-alpha related apoptosis induced-ligand (TRAIL) in macrophages killing HepG2 cells[J].Chinese Journal of Hepatology, 2005, 13(9):689-691.
[48] YI L, SUN C, WU J, et al.Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus[J].Journal of Fish Diseases, 2022, 45(6):833-846.
[49] BURGALETTO C, PLATANIA C, DI BENEDETTO G, et al.Targeting the miRNA-155/TNFSF10 network restrains inflammatory response in the retina in a mouse model of Alzheimer's disease[J]. Cell Death & Disease, 2021, 12(10):905.
[50] CHO Y S, CHALLA S, CLANCY L, et al.Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation[J].Immunology, 2010, 130(4):504-515.
[51] CANTARELLA G, DI BENEDETTO G, PUZZO D, et al.Neutralization of TNFSF10 ameliorates functional outcome in a murine model of Alzheimer's disease[J]. Brain, 2015, 138(Pt 1):203-216.
[52] ADACHI T, SUGIYAMA N, GONDAI T, et al.Blockade of death ligand TRAIL inhibits renal ischemia reperfusion injury[J]. Acta Histochemica et Cytochemica, 2013, 46(6):161-170.
[53] HAN L H, SUN W S, MA C H, et al.Detection of soluble TRAIL in HBV infected patients and its clinical implications[J]. World Journal of Gastroenterology, 2002, 8(6):1077-1080.
[54] DUNN C, BRUNETTO M, REYNOLDS G, et al.Cytokines induced during chronic Hepatitis B virus infection promote a pathway for NK cell-mediated liver damage[J]. The Journal of Experimental Medicine 2007, 204(3):667-680.
[55] LI X, YANG H, WU S, et al.Suppression of PTPN6 exacerbates aluminum oxide nanoparticle-induced COPD-like lesions in mice through activation of STAT pathway[J].Particle and Fibre Toxicology, 2017, 14(1):53.
[56] NG T H, CHIANG Y A, YEH Y C, et al.Review of Dscam-mediated immunity in shrimp and other arthropods[J].Developmental & Comparative Immunology, 2014, 46(2):129-138.
[57] CHANG C C, TUNG C H, CHEN C W, et al.SUMOgo:Prediction of sumoylation sites on lysines by motif screening models and the effects of various post-translational modifications[J]. Scientific Reports, 2018, 8(1):15512.
[58] CAO Y, HUANG W, WU F, et al.ZFP36 protects lungs from intestinal I/R-induced injury and fibrosis through the CREBBP/p53/p21/Bax pathway[J].Cell Death & Disease, 2021, 12(7):685.
[59] DHO S E, FRENCH M B, WOODS S A, et al.Characterization of four mammalian numb protein isoforms.Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain[J].Journal of Biological Chemistry, 1999, 274(46):33097-33104.
[60] LAU C I, BARBARULO A, SOLANKI A, et al.The kinesin motor protein Kif7 is required for T-cell development and normal MHC expression on thymic epithelial cells (TEC) in the thymus[J].Oncotarget, 2017, 8(15):24163-24176.
[61] JIAN D, WANG Y, JIAN L, et al.METTL14 aggravates endothelial inflammation and atherosclerosis by increasing FOXO1 N6-methyladeosine modifications.[J]. Theranostics, 2020, 10(20):8939-8956.
[62] MENG J, JIANG J J, ATSUMI T, et al.Breakpoint cluster region-mediated inflammation is dependent on casein kinase Ⅱ[J]. Journal of Immunology, 2016, 1978(8):3111-3119.

Screening of Genes Related to Bursa of Fabricius Injury Induced by LPS in Magang Geese

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Rui, SHENG Hui, GUO Yanyan, ZHANG Yuxin, YAO Dawei, GUO Xiaofei, RUAN Weibin, ZHANG Xiaosheng. Research Progress on Immune Stress of LPS [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 946-958.
[2]	CHANG Xinxin, HUANG Xin, FAN Gang, YANG Jiaxin, ZHANG Weiwei, YANG Qingzhu, TIAN Zhe. Inhibitory Effect of Cannabidiol on LPS-induced Inflammatory Response and Apoptosis of Mouse Mammary Epithelial Cells [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 60-69.
[3]	XU Haotian, YU Yuetong, LI Jing, MA Zhiyuan, YANG Bin, WANG Zekun, TUO Haixin, QI Meng. Comparative Analysis of miRNA Expression Profiles in Bovine Mammary Epithelial Cells Treated with Lipopolysaccharide and Calcium Ions [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4066-4079.
[4]	FANG Renlai, CHEN Jingyi, LI Hanglin, LIU Qing, CHEN Qi, HU Tingjun. Protective Effect of Cangpu Wumei Powder on Diarrhea Mice Induced by Lipopolysaccharide [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4143-4152.
[5]	HE Jiayu, YANG Mingqi, WU Jiao, YANG Yu, YAO Huan, YI Jine, KONG Li. Establishment and Index Evaluation of Inflammatory Bowel Disease Model in C57BL/6 Mice [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4153-4163.
[6]	ZHANG Xiaohan, SUN Lanyuan, SONG Zhuan, HOU Yongqing, WU Tao. Effect of Chebuli Extract on Intestinal and Liver Damage Induced by Lipopolysaccharide in Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3267-3278.
[7]	HU Mingxia, DENG Yanping, HE Yongyun, YAO Yue, MO Xiaodan, HUANG An, YANG Xiufen. Protective Effect of Alcohol Extract from Curcuma kwangsiensis on Lung Injury Induced by LPS in Mice [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3652-3661.
[8]	WANG Shunbo, WANG Hao, LIANG Yunyi, WANG Wenjing, LI Zicong, XU Zheng. Effects of Feeding Risperidone During Late Pregnancy on Mammary Gland Development of Maternal Mice and Growth of Offspring Mice [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2489-2500.
[9]	LU Qingwei, TANG Sen, LI Bin, QIN Chongkai, LIU Wenna, PU Xue, WANG Yaqian, WU Cuiling, FU Xuefeng. Screening of circRNA Related to Secondary Hair Follicle Cycle Development in Jiangnan Cashmere Goats Based on Transcriptome Sequencing [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 1793-1806.
[10]	FENG Xiaomeng, GAO Chao, TAO Xinlei, LI Xiaofang, LYU Xiaoping, GAO Xueli, ZHAO Yi, LI Yanan, LIU Chaonan. Study on the Regulatory Role of the HIF-1α Signaling Pathway on Lipopolysaccharide-induced Inflammation in Chicken Macrophages [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2071-2080.
[11]	BAO Hua, HU Chunli, AN Yanhao, ZHANG Chunhua, MA Yanfen. Effects of EGCG on Inflammatory Injury and Apoptosis of BMECs Induced by LPS [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2122-2131.
[12]	LIU Yingkun, GUO Wei, FU Zengyu, SUN Meng, LI Haojia, ZHOU Haizhu. Effects of Ferulic Acid on Growth Performance and Intestinal Anti-oxidant Indicators of Jilin White Geese Under Lipopolysaccharide Stress [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(2): 521-529.
[13]	MA Yuan, JIN Haoyan, WANG Nana, LI Qihan, ZHANG Lingkai. Progress in the Application of Single-cell Transcriptome Sequencing Technology in Spermatogenesis [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4400-4409.
[14]	SUN Xiangli, SHI Ziyao, LI Keke, LIU Yuxuan, WANG Ruolin, LIU Yang, WANG Yanbin. Effect of Feeding Clostridium butyricum on the Morphology and Transcriptomics of Cecum Mucosa in Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(9): 3584-3593.
[15]	LIU Ling, WANG Shengnan, WANG Dandan, MA Yuehui, JIANG Lin, CUI Kai. Effect and Molecular Mechanism of Zbed6 Gene Knockout on the Growth and Development of Skeletal Muscle in Mice [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(9): 3641-3651.