药用槐花抗炎活性的网络药理学分析

doi:10.16431/j.cnki.1671-7236.2022.09.036

Abstract

Abstract: 【Objective】 The aim of this study was to explore the anti-inflammatory mechanism of the main active components of Sophora japonica L.(S.japonica L.) based on network pharmacology.【Method】 The effective components and targets of S.japonica L.were screened from the TCMSP database and constructed an active component-target network diagram using the Uniprot database and the Cytoscape 3.6.1 software.Combined with the NCBI, GeneCards and OMIM databases to search the anti-inflammatory related targets of S.japonica L., the core target genes were uploaded to the STRING platform to build a protein-protein interaction network (PPI). DAVID database was used to analyze key targets by GO function and KEGG pathway enrichment.【Result】 The results showed that there were six active components in S.japonica L.including quercetin, isorhamnetin, kaempferol, beta-sitosterol, N-[6-(9-acridinylamino) hexyl] benzamide and quercetin-3-methyl ether.It could act on 152 anti-inflammatory targets including IL10, NFKBIA, ICAM1, MMP2, BCL2L1, STAT1, VCAM1, IFNG, MAPK14 and IL2.There were 16 items related to biological processes, including positive regulation of transcription from RNA polymerase Ⅱ promoter, transcription DNA-templated and gene expression, negative regulation of apoptotic process, inflammatory response and cellular response to lipopolysaccharide (LPS), etc.;6 items related to molecular function, including enzyme binding, transcription factor binding, protein binding, etc.;2 items related to cellular components, including extracellular space and cytosol. And 21 related signaling pathways, including TNF signaling pathway, Toll-like receptor signaling pathway, T cell receptor signaling pathway, NOD-like receptor signaling pathway, cancer pathway, hepatitis B, HTLV-Ⅰ infection and influenza A, etc.【Conclusion】 The results showed S.japonica L. might act on key targets such as quercetin, isorhamnetin, kaoneferol, beta-sitosterol and other active ingredients, played an anti-inflammatory role mainly through TNF, Toll-like receptor, T-cell receptor and NOD like receptor signaling pathways acting on RB1, CDKN1A, IKBKB, CHU, IL2, CXCL10 and other key targets.The results indicated that the anti-inflammatory mechanism of S.japonica L. might involve in multiple components, targets and pathways.

Key words: network pharmacology; Sophora japonica L.; anti-inflammatory activity; targets; signaling pathway

CLC Number:

S853.74

FAN Qiuyu, WU Jianwen, LI Huanrong. Network Pharmacology Analysis of Anti-inflammatory Activity of Medicinal Sophora japonica L.[J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(9): 3622-3632.

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Network Pharmacology Analysis of Anti-inflammatory Activity of Medicinal Sophora japonica L.

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