CRISPR/dCas9技术在基因表达调控中的研究进展

doi:10.16431/j.cnki.1671-7236.2022.01.006

Abstract

Abstract: CRISPR/dCas9 is a system for regulating genome transcription and epigenetic modification based on the transformation and upgrading of the CRISPR/Cas9 gene editing system. It not only inherits the target accuracy of the CRISPR/Cas9 system, at the same time, it also showed a good effect. In this system, the dCas9 protein retains the ability of the Cas9 protein to bind DNA and the cleavage function no longer exists. Coupling dCas9 protein with different activation and inhibitory effector domains and epigenetic regulation enzymes makes it possible to precisely regulate gene expression and epigenetic modification. Epigenetic modification processes such as histone acetylation, histone methylation and DNA methylation are the basis of gene expression and have made a huge contribution to the entire life process. At the same time, epigenetics is causally related to many diseases and cancers. Different epigenetic modification systems based on the CRISPR/dCas9 system have unlimited future and research value in the field of human disease treatment and cancer research. Therefore, this article briefly introduces the discovery process and the principle of action of the CRISPR/Cas9 system. It mainly summarizes the application and optimization process of different regulatory systems based on the CRISPR/dCas9 system in the regulation of gene expression and epigenetic regulation. It hopes to provide some references for scientific research workers engaged in related fields.

Key words: CRISPR/Cas9; dCas9; activation; interference; epigenetic modification

CLC Number:

YANG Sha, HAO Haisheng, DU Weihua, PANG Yunwei, ZHAO Shanjiang, ZOU Huiying, ZHU Huabin, YANG Yuze, ZHAO Xueming. Research Progress of CRISPR/dCas9 Technology in Gene Expression Regulation[J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(1): 53-59.

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Research Progress of CRISPR/dCas9 Technology in Gene Expression Regulation

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