代谢组学技术在有毒有害物质检测应用中的研究进展

doi:10.16431/j.cnki.1671-7236.2019.06.036

Abstract

Abstract:

Some toxic and harmful substances can lead to huge risks of food safety such as pathogenic microorganisms,biotoxins,pesticides,veterinary drugs and additives residues.Nowadays,monitoring of toxic and hazardous substances still faces enormous challenges.Metabolomics techniques detect metabolically changes in organisms which are stimulated or interfered by toxic and hazardous substances,then it will perform qualitative and quantitative analysis of the data and obtain highly sensitive and specific metabolomics projection,which makes it suitable for simultaneous monitoring of different trace toxicants.Metabolomics technology can reflect possible biomarkers in the process of toxicant metabolism,and provide references for further researches on toxic metabolic processes and toxicological mechanisms.Therefore,the metabolomics method represented by chromatographic mass spectrometry,nuclear magnetic resonance and other methods has gradually become a key technology for the detection of toxic and hazardous substances,and it provides a basis for risk assessment of toxic and hazardous substances.The discovery of various biomarkers and the optimization of detection methods will promote the practical application of metabolomics technology in simultaneous monitoring of toxicant.The generation and development of metabolomics technology were introduced,and the researches of metabonomics methods and biomarkers of metabolites were summarized in this paper in order to provide references for the further application of metabolomics technology in the detection of toxic and harmful substances.

Key words: metabolomics; toxic and harmful substances; biomarkers; residue

CLC Number:

R284

OU Yahong, WANG Yuke, ZHANG Liyun, WANG Yanxin, PENG Dapeng, XIE Shuyu, HUANG Lingli, PAN Yuanhu, CHEN Dongmei, WANG Xu, WANG Yulian, TAO Yanfei. Research Progress on Metabonomics in the Detection of Toxic and Harmful Substances[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(6): 1865-1872.

References

[1] 王晶,汤柳英,杨杏芳,等.代谢组学技术及其研究进展[J].中国卫生检验杂志,2013,23(4):1046-1050. WANG J,TANG L Y,YANG X F,et al.Research progress of metabonomics[J].Chinese Journal of Health Laboratory Technology,2013,23(4):1046-1050.(in Chinese)
[2] NICHOLSON J K,LINDON J C,HOLMES E.‘Metabonomics’:Understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189.
[3] FIEHN O,KOPKA J,DORMANN P,et al.Metabolite profiling for plant functional genomics[J].Nature Biotechnology,2000,18(11):1157-1161.
[4] DETTMER K,HAMMOCK B D.Metabolomics-A new exciting field within the "omics" sciences[J].Environmental Health Perspectives,2004,112(7):A396-A397.
[5] TAYLOR J,BUCKINGHAM M J,SADLER P J.Application of metabolomics to plant genotype discrimination using statistics and machine learning[J].Bioinforrnatics,2002,18:241-248.
[6] 刘思洁,吴永宁,方赤光.代谢组学技术在食品安全中的应用[J].食品安全质量检测学报,2014,5(4):1081-1086. LIU S J,WU Y N,FANG C G,et al.Review on the application of metabonomics approach in food safety[J].Journal of Food Safety & Quality,2014,5(4):1081-1086.(in Chinese)
[7] 吕尚,杨世林,饶毅,等.代谢组学及其相关技术在中药研发领域的应用进展[J].中国中药杂志,2018,43(21):4182-4191. LYU S,YANG S L,RAO Y,et al.Application of metabolomics and related technologies in research and development field of traditional Chinese medicine[J].China Journal of Chinese Materia Medica,2018,43(21):4182-4191.(in Chinese)
[8] GEORGIOS A,THEODORIDI S,HELEN G,et al.Liquid chromatography mass spectrometry based global metabolite profiling:A review[J].Analytica Chimica Acta,2012,711:7-16.
[9] BARBAS C,MORAESA E P,VILLASENORA A.Capillary electrophoresis as a metabolomics tool for nontargeted fingerprinting[J].Journal of Pharmaceutical and Biomedical Analysis,2011,55(4):823-831.
[10] THOMPSON D F,MICHICHOPOULOS F,SMITH C J,et al.Profiling biological samples using ultraperformance liquid chromatography-inductively coupled plasma mass spectrometry (UPLC-ICP-MS) for the determination of phosphorus and sulfur containing metabolites[J].Molecular Biosystems,2011,7(4):1149.
[11] DAI S,SHANG Z,WANG F,et al.Novelty application of multiomics correlation in the discrimination of sulfur fumigation and nonsulfurfumigation Ophiopogonis Radix[J].Scientific Reports,2017,7(1):9971.
[12] PINTO J.Metabonomics of the blood of pregnant women for diagnosis of prenatal disorders[D].Portugal:Universidade De Aveiro,2010.
[13] 郝杰,姜洁,毛婷,等.代谢组学技术在食品安全风险监测中的研究进展[J].食品安全质量检测学报,2017,8(7):2587-2595. HAO J,JIANG J,MAO T,et al,Research progress of metabonomics in food safety risk monitoring[J].Journal of Food Safety and Quality,2017,8(7):2587-2595.(in Chinese)
[14] 董蓉,吴清平,张菊梅,等.食源性致病沙门氏菌血清型生物标志物的研究[J].现代食品科技,2017,33(5):288-292. DONG R,WU Q P,ZHANG J M,et al.Study on biomarkers of foodborne pathogenic Salmonella serotypes[J].Modern Food Science and Technology,2017,33(5):288-292.(in Chinese)
[15] LI Z C,ZHANG Z D,LI L X,et al.Developing generic and targeted LC-MS approaches for E.coli central carbon metabolomic study[A].Proceedings of the 10th National Conference on Biomedical Chromatography and Related Technology[C].2014.
[16] 孙璐璐.大鼠革兰阴性菌和革兰阳性菌感染脓毒症模型的构建及代谢组学研究[D].上海:第二军医大学,2010. SUN L L.A metabonomic approach for the early diagnosis and identification of experimental sepsis induced by Gram-negative and Gram-positive bacterium in rats[D].Shanghai:The Second Military Medical Universuty,2010.(in Chinese)
[17] 陈雪,倪鹏,喻勇新,等.李斯特属细菌特征挥发性代谢物的鉴定分析[J].食品科学,2013,34(10):231-237. CHEN X,NI P,YU Y X,et al.Characterization of volatile metabolites of Listeria spp.strains[J].Food Science,2013,34(10):231-237.
[18] 单晓雪,胡建蓉,熊升伟.我国粮食作物中的黄曲霉毒素的研究进展[J].食品安全导刊,2015,18:47-48. SHAN X X,HU J R,XIONG S W,et al.Research progress of aflatoxins in food crops in China[J].China Food Safety Magazine,2015,18:47-48.(in Chinese)
[19] 夏骏,李勇,徐国茂,等.动物源性食品中赭曲霉毒素A毒性和检测方法研究进展[J].江西畜牧兽医杂志,2015,2:4-7. XIA J,LI Y,XU G M,et al.Research progress of toxicity and detection methods of ochratoxin A in animal-derived foods[J].Jiangxi Journal of Animal Husbandry and Veterinary Medicine,2015,2:4-7.(in Chinese)
[20] YANG S,ZHANG H,DE S S,et al.In vitro and in vivo metabolism of ochratoxin A:A comparative study using ultra performance liquid chromatography quadrupole/time of flight hybrid mass spectrometry[J].Analytical & Bioanalytical Chemistry,2015,407(13):3579-3589.
[21] 林妮妮,郭磊,陈佳,等.基于高效液相色谱-串级质谱法研究肝微粒体中T-2毒素代谢的种属差异性[J].中国药理学与毒理学杂志,2017,31(7):754-759. LIN N N,GUO L,CHEN J,et al.Species difference of T-2 toxin metabolism in liver microsomes by high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Pharmacology and Toxicology,2017,31(7):754-759.(in Chinese)
[22] 柳洁,黄海燕,曾灼祥,等.大鼠血清和尿液中4种鹅膏肽类毒素代谢特征的超高效液相色谱-四极杆飞行时间串联质谱分析[J].中国卫生检验杂志,2018,28(16):1941-1946. LIU J,HUANG H Y,ZENG Z X,et al.Analysis of four amatoxins and phallotoxins content in rat serum and urine by ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry[J].Chinese Journal of Health Laboratory Technology,2018,28(16):1941-1946.(in Chinese)
[23] 吴海燕,郭萌萌,郑关超,等.氮杂螺环酸毒素的生态分布、蓄积代谢与检测技术监控研究进展[J].中国渔业质量与标准,2018,8(1):7-15. WU H Y,GUO M M,ZHENG G C,et al.Research development on azaspiracids ecological distribution,edible safety risks monitoring and detection methods[J].Chinese Fishery Quality and Standards,2018,8(1):7-15.(in Chinese)
[24] REHMANN N,HESS P,QUILLIAM M A.Discovery of new analogs of the marine biotoxin azaspiracid in blue mussels (Mytilus edulis) by ultra-performance liquid chromatography/tandem mass spectrometry[J].Rapid Communications in Mass Spectrometry,2008,22(4):549-558.
[25] CANAS I R,O'CALLAGHAN K,MORONEY C,et al.The development of a rapid method for the isolation of four azaspiracids for use as reference materials for quantitative LC-MS-MS methods[J].Analytical and Bioanalytical Chemistry,2010,398(3):1477-1491.
[26] CHEVALLIER O P,GRAHAM S F,ALONSO E,et al.New insights into the causes of human illness due to consumption of azaspiracid contaminated shellfish[J].Scientific Report,2015,5:9818.
[27] 张艳欣,杨鑫,谢冰洁,等.基于HPLC/Q-TOF MS的4种农药联合暴露人群的代谢组学研究[J].分析测试学报,2016,35(5):501-507. ZHANG Y X,YANG X,XIE B J,et al.Metabolomics tools for describing complex pesticide exposure in occupational population by HPLC/Q-TOF MS[J].Journal of Instrumental Analysis,2016,35(5):501-507.(in Chinese)
[28] MAHDAVI V,FARIMANI M M,FATHI F,et al.A targeted metabolomics approach toward understanding metabolic variations in rice under pesticide stress[J].Analytical Biochemistry,2015,478:65-72.
[29] XU H D,WANG J S,LI M H,et al.(1)H-NMR based metabolomics approach to study the toxic effects of herbicide butachlor on goldfish (Carassius auratus)[J].Chemosphere,2015,159:69-80.
[30] WANG P,WANG H P,XU M Y,et al.Combined subchronic toxicity of dichlorvos with malathion or pirimicarb in mice liver and seruma metabonomic study[J].Food & Chemical Toxicology,2014,70(2):222-230.
[31] 王世成,李国琛,王颜红,等.代谢组学方法研究奶牛注射氯霉素后牛奶中的生物标志物[J].分析化学,2010,38(8):1199-1202. WANG S C,LI G C,WANG Y H,et al.Study of potential biomakers in milk from cows treated with chloram phenicolby metabonomic approach[J].Chinese Journal of Analytical Chemistry,2010,38(8):1199-1202.(in Chinese)
[32] HAN H,XIAO H,LU Z.Short term toxicity assessments of an antibiotic metabolite in Wistar rats and its metabolomics analysis by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry[J].Toxicology & Applied Pharmacology,2016,293(6):1-9.
[33] ARIAS M,CHEXALLIER O P,GRAHAM S F,et al.Metabolomics reveals novel biomarkers of illegal 5-nitromimidazole treatment in pigs.Further evidence of drug toxicity uncovered[J].Food Chemistry,2016,199:876-884.
[34] DE SOTTO R,MEDRIANO C,CHO Y,et al.Significance of metabolite extraction method for evaluating sulfamethazine toxicity in adult zebrafish using metabolomics[J].Ecotoxicology & Environmental Safety,2016,127:127-134.
[35] WU Y,ZHANG Y.Analytical chemistry,toxicology,epidemiology and health imp-act assessment of melamine in infant formula:Recent progress and developments[J].Food & Chemical Toxicology,2013,56(2):325-335.
[36] DUAN H,GUAN N,WU Y,et al.Identification of biomarkers for melamine-induced nephrrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry(U-HPLC-Q-TOF/MS)[J].Journal of Chromatography B,2011,879(30):3544-3550.
[37] XIE G,ZHENG X,QI X,et al.Metabonomic evaluation of melamine induced acute renal toxicity in rats[J].Technical Notes Journal of Proteome Research,2010,9(1):125-133.
[38] SCHNACKENBERG L K,SUN J,PENCE L M,et al.Metabolomics evaluation of hydroxyproline as a potential marker of melamine and cyanuric acid nephrotoxicity in male and female Fischer F344 rats[J].Food & Chemical Toxicology,2012,50(11):3978-3983.
[39] COURANT F,PINEL G,BICHON E,et al.Development of a metabolomic approach based on liquid chromatography-high resolution mass spectrometry to screen for clenbuterol abuse in calves[J].Analyst,2009,134(8):1637-1646.
[40] LI G,FU Y,HAN X,et al.Metabolomic investigation of porcine muscle and fatty tissue after clenbuterol treatment using gas chromatography/mass spectrometry[J].Journal of Chromatography A,2016,1456:242-248.
[41] WU Y,BI Y,BINGGA G,et al.Metabolomic analysis of swine urine treated with β₂-agonists by ultrahigh performance liquid chromatography-quadrupole time-of-flight masss-pectrometry[J].Journal of Chromatography A,2015,1400:74-81.
[42] 张可冬,招启文,陈晓,等.超高效液相色谱-串联质谱法测定肉制品中的克伦特罗[J].食品安全质量检测学报,2018,9(5):1097-1103. ZHANG K D,ZHAO Q W,CHEN X,et al.Determination of clenbuterol in meat products by ultra performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality,2018,9(5):1097-1103.(in Chinese)
[43] REGAL P,ANIZAN S,ANTIGNAC J P,et al.Metabolomic approach based on liquid chromatography coupled to high resolution mass spectrometry to screen for the illegal use of estradiol and progesterone in cattle[J].Analytica Chimica Acta,2011,700(1-2):16-25.
[44] REGAL P,SEIGAS J A,CEPEDA A,et al.Structure elucidation and HPLC-MS/MS determination of a potential biomarker for estradiol administration in cattle[J].Analytical and Bioanalytical Chemistry,2013,405(29):9537-9546.
[45] 杜航,龚进,代华,等.慢性砷中毒患者头发代谢物特征的代谢组学研究[J].环境与职业医学,2018,35(2):163-167. DU H,GONG J,DAI H,et al.Metabonomics study on metabolic profile of hair samples from chronic arsenic poisoning patients[J].Journal of Environmental & Occupational Medicine,2018,35(2):163-167.(in Chinese)
[46] 王晓飞,蒋守芳,张维冰,等.利用代谢组学研究大气细颗粒物的生殖毒性效应[J].分析化学,2017,45(5):633-640. WANG X F,JIANG S F,ZHANG W B,et al.Study on reproductive toxicity of fine particulate matter by metabolomics[J].Chinese Journal of Analytical Chemistry,2017,45(5):633-640.(in Chinese)

Research Progress on Metabonomics in the Detection of Toxic and Harmful Substances

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Xiaotong, GE Bingjie, QIU Qian, LIU Xinman, YU Minghong, ZHANG Xuemei. Application of Bioinformatics in Toxicity Mechanism of Aflatoxin B₁ [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1639-1650.
[2]	CHEN Xiuying, LI Aijun, LI Mingyang, ZHANG Caihua, CUI Yanan, ZHOU Lina, GU Shouguo, ZHOU Yancheng. Research Progress on Pretreatment and Detection of Veterinary Drug Residues in Food of Animal Origin [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1914-1923.
[3]	AN Zhaoxiang, XUN Wenjuan, ZHOU Hanlin, SHI Liguang. Effects of Heat Stress on Semen Antioxidant Capacity and Seminal Plasma Metabolomics in Hainan Black Goats [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1191-1201.
[4]	LUO Qin, LIU Dingyu, LIU Baoling, QIAO Changhong, CHEN Xiangyu, HE Zhenwen, WANG Xiaohu, CHEN Jing, ZHANG Pian, HUANG Yuan, WANG Gang, LIU Hao, CAI Rujian. Research Progress on Antiviral Effect of Traditional Chinese Medicine Analyzed from Different Omics Perspectives [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 554-561.
[5]	QIAO Changhong, CHEN Xiangyu, LIU Baoling, LUO Qin, LIU Dingyu, HE Zhenwen, WANG Xiaohu, CHEN Jing, ZHANG Pian, HUANG Yuan, BAI Aiquan, WANG Gang, CAI Rujian. Research Progress on the Antimicrobial Mechanism of Traditional Chinese Medicine Through a Multi-omics Perspective [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 52-59.
[6]	FENG Yi, YANG Yunyun, LUO Yongrong, WU Peng, WANG Yan, YANG Ying, CHEN Jiangfeng, JIANG Haibo, WEN Ming. Effect of Dietary Addition of Rosa roxburghii Tratt. Residue on Intestinal Histomorphology,Antioxidant Capacity,Immunity and Metabolomics in Common Carp (Cyprinus carpio) [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 80-93.
[7]	LI Qilong, SHI Hanjing, CHEN Sisi, XU Junfei, ZHOU Wenyue, GONG Saibin, LUO Jie, YANG Feilai, LI Fengna, GUO Qiuping. Effects of Dietary Fermented Shiitake Residues Supplementation on Growth Performance,Meat Quality and Flavor Substances of Finishing Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 182-194.
[8]	HE Jianjian, GAO Huixia, SUN Chenxu, YAO Haibo, XIE Yaodi, YU Aihuan, HU Jinsheng, WANG He, DUAN Yueyan, TANG Defu, LIU Wangjing. Effect of Allium mongolicum Regel Powder on Nutrient Apparent Digestibility and Plasma Metabolomics in Angus Calves [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3780-3793.
[9]	GUO Tongjun, XUE Yuang, GAO Li, HAN Xubiao, SHI Yingwu, SANG Duanji. Analytical Testing for Pesticide Residues,Heavy Metals and Fungal Toxins in Cotton Straw [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 1880-1892.
[10]	ZHANG Qiang, LEI Xiaowen, LIAN Hai, SHI Xiaosheng, HUANG Zhiwen, ZHONG Yunping. Study on the Quality and Microbial Diversity of Mixed Silage Between Distillers Grains and Navel Orange Residue [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1456-1465.
[11]	ZHANG Xingyan, LAN Haien, YI Xianfeng, YANG Kai, ZHU Wen, LAN Xi, ZHOU Junhua, CHEN Zhonghua. Metabolomics Analysis of Fermented Elephant Grass and It’s Effects on the Growth Performance and Serum Biochemical Indicators of Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(11): 4802-4811.
[12]	WU Zhanyue, GUO Yanhong, ZHUANG Lei, WANG Ning, LU Zhentao, REN Wenwen, XU Tong, WANG Qimeng, NIU Zhili, CAO Junting, WU Yongbao, WU Sen, WEN Zhiguo. Evaluation of Nutrient Utilization of Sugar Residue and Soy Sauce Residue in Meat Ducks [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(11): 4833-4841.
[13]	CHANG Anqi, MAO Shuaixiang, LIU Guangbin. Research Progress on Feed Utilization of Grape Residueand and Its Application in Sheep Production [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(11): 4842-4850.
[14]	XUAN Yi, ZHANG Yu, HUANG Xiaoning, JIANG Xinyu, WEI Kexin, WANG Xiaotong, XIE Xiaocheng, LI Chengui. Research Progress of Molecular Imprinting Sensors in the Field of Veterinary Drug Residue Analysis [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(1): 427-433.
[15]	HOU Zhiming, ZHANG Lei, HE Yibo, ZHANG Junpeng, ZHANG Shuyi. Research Progress of Omics Techniques in the Field of Subclinical Mastitis in Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(9): 3510-3518.