转基因猪中抗生素标记基因neo漂移风险评估

doi:10.16431/j.cnki.1671-7236.2015.10.031

Abstract

Abstract: This study was designed to study the possibility of gene drift of the antibiotic marker gene neo in transgenic pigs.First,dominance and recessiveness of neo gene in 6 piglets of F1 were identified using Southern blotting.It was found that 3 piglets were positive transgenic piglets and the other 3 piglets were non-transgenic piglets.The neo gene in blood and digestive tract tissue of experimental piglets were detected using PCR.The result showed that no gene drift was showed in blood and digestive tract tissues of experimental piglets.Then PCR technology was used to detect neo gene in intestinal bacteria of experimental piglets.The result showed that neo gene did not exist in the genome of intestinal bacteria.This study suggested that no gene drift was found in blood,intestinal bacteria and digestive tract tissues of piglets.

Key words: transgenic piglets; neo gene; gene drift

CLC Number:

WANG Qing-qing, GAO Peng-fei, LI He-gang, MA De-zun, CAI Chun-bo, QIAN Li-li, JIANG Sheng-wang, CUI Wen-tao. Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(10): 2720-2725.

References

[1] Yenofsky R L,Fine M,Pellow J W.A mutant neomycin phosphotransferase Ⅱ gene reduces the resistance of transformants to antibiotic selection pressure[J]. Proceedings of the National Academy of Sciences,1990,87(9):3435-3439.
[2] Anfossi L,Tozzi C,Giraudi G,et al.Evaluation of procedures for the extraction and purification of neomycin phosphotransferase Ⅱ from a genetically modified agrobacterium[J].Annali Di Chimica,2004,94(1-2):93-99.
[3] 王关林,方宏筠.植物基因工程[M].第2版.北京:科学出版社,2002.
[4] Miki B,McHugh S.Selectable marker genes in transgenic plants:Applications,alternatives and biosafety[J].Journal of Biotechnology,2004,107(3):193-232.
[5] 崔文涛,单同领,李奎.转基因猪的研究现状及应用前景[J].中国畜牧兽医,2007,34(4):58-62.
[6] 耿彩霞,狄冉,储明星,等.转基因动物的制备方法及应用评述[J].中国畜牧兽医,2010,37(6):130-133.
[7] Fuchs R L,Heeren R A,Gustafson M E,et al.Purification and characterization of microbially expressed neomycin phosphotransferase Ⅱ (NPTⅡ) protein and its equivalence to the plant expressed protein[J].Nature Biotechnology,1993,11(12):1537-1542.
[8] Fuchs R L,Ream J E,Hammond B G,et al.Safety assessment of the neomycin phosphotransferase Ⅱ (NPTⅡ) protein[J]. Bio Technology,1993,11(13):1543-1547.
[9] Hoffmann T,Golz C,Schieder O.Foreign DNA sequences are received by a wild-type strain of Aspergillus niger after co-culture with transgenic higher plants[J].Current Genetics,1994,27(1):70-76.
[10] Nielsen K M,van Elsas J D,Smalla K.Transformation of Acinetobacter sp.strain BD413 (pFG4ΔnptⅡ) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants[J].Applied and Environmental Microbiology,2000,66(3):1237-1242.
[11] Bennett P M,Livesey C T,Nathwani D,et al.An assessment of the risks associated with the use of antibiotic resistance genes in genetically modified plants:Report of the working party of the British society for antimicrobial chemotherapy[J].Journal of Antimicrobial Chemotherapy,2004,53(3):418-431.
[12] Daniell H,Muthukumar B,Lee S B.Marker free transgenic plants:Engineering the chloroplast genome without the use of antibiotic selection[J].Current Genetics,2001,39(2):109-116.
[13] 许宏涛,张秀珍.医院感染铜绿假单胞菌多重耐药机制的研究[J].中国抗感染化疗杂志,2005,5(3):141-145.
[14] Chambers S P,Prior S E,Barstow D A,et al.The pMTL nic-cloning vectors.Ⅰ.Improved pUC polylinker regions to facilitate the use of sonicated DNA for neclotide sequencing[J].Gene,1988,68(1):139-149.
[15] 樊龙江,周雪平.转基因作物安全性争论与事实[M].北京:中国农业出版社,2000.
[16] Nielsen K M,Bones A M,Smalla K,et al.Horizontal gene transfer from transgenic plants to terrestrial bacteria——A rare event[J]. FEMS Microbiology Reviews,1998,22(2):79-103.
[17] Schlüter K,Fütterer J,Potrykus I."Horizontal" gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs——If at all——At an extremely low frequency[J].Nature Biotechnology,1995,13(10):1094-1098.

Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs

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