Abstract: In order to determine the reason of gibbon's death in a safari park of Guizhou province, the infected gibbons from this safari park were detected using epidemiological survey, autopsy pathological observation, anatomic pathological diagnosis and RT-PCR method.The results showed that from epidemiological survey and autopsy pathological observation, we primarily diagnosed that the gibbons were suspectedly infected by influenza virus, Mycoplasma and bacteria.The RT-PCR/PCR results confirmed that the gibbons were mixed infected by influenza virus and Mycoplasma, and the bacterium was confirmed as Pasteurella multocida by bacteria culture and isolation, biochemical character identification and animal pathogenicity test.All above test results showed that mixed infection of influenza virus, Mycoplasma and Pasteurella multocida were the cause of the gibbon's death in this safari park.According to the results of diagnosis and drug sensitivity test, we made the immune prevention and treatment measures.

Key words: gibbon; influenza virus; Mycoplasma; Pasteurella multocida; mixed infection; diagnosis and treatment