牛病毒性腹泻病毒间接ELISA方法的建立

doi:10.11843/j.issn.1671-7236.2015.03.008

Abstract

Abstract: Persistent infection and immune tolerance were important features of bovine viral diarrheas,and also the prevention and control difficulties of the disease.In the experiment,bovine viral diarrhea virus (BVDV) species,CP (BVDV OregonC24 strain) type and NCP (BVDV yak strain) type were inactivated,concentrated as antigen and immuned rabbits to prepare high serum,respectively.With BVDV full viral proteins as antigens,we established an indirect ELISA detection method for BVDV.We detected the high serum and explored the antigen immunogenicity differences of CP type BVDV and NCP type BVDV using the established indirect ELISA method.The results showed that high serum titer of CP type BVDV was higher than that of NCP type BVDV,with an average high of about 35%.This result showed that the immunogenicity of CP type BVDV was superior to the NCP type BVDV,and the serum titer of CP type BVDV and NCP type BVDV had significant differences.This result would provide the guidance of the theory for the selection and production of vaccine strain in practice.

Key words: bovine viral diarrhea virus (BVDV); indirect ELISA; immunogenicity

CLC Number:

S852.4

LI Wen-wen, LIU Qing, LIU Ya-gang. Establishment of Indirect ELISA Method of Bovine Viral Diarrhea Virus[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(3): 558-562.

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Establishment of Indirect ELISA Method of Bovine Viral Diarrhea Virus

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