丁酸钠对鱼类肠道消化吸收的影响研究进展

doi:10.16431/j.cnki.1671-7236.2022.01.014

摘要/Abstract

摘要： 与畜禽等动物相比，鱼类消化系统不健全，消化酶活性低，而植物性原料中常含有抗营养因子和毒性成分等，易导致肠道损伤及肠炎等疾病。丁酸等短链脂肪酸可显著改善鱼类生长性能，增强鱼体健康及抗氧化和抗应激能力。因丁酸气味不佳，影响动物适口性，丁酸钠作为丁酸的替代物，呈固态且不易挥发，易于添加使用。丁酸钠可促进肠道上皮细胞发育，改善肠道形态结构，保持肠道黏膜屏障完整，对损伤的肠道黏膜加以修复，刺激上皮细胞内mRNA和蛋白质合成，加速肠上皮细胞增殖，增加绒毛高度，促进消化吸收。丁酸钠能破坏有害菌DNA结构，蛋白质不能合成，使有害菌不能进行正常分裂，抑制肠道有害菌的生长繁殖，促进有益菌群的增殖，维持肠道菌群平衡，菌群多样性显著提高。丁酸钠可通过刺激消化酶的分泌来改善鱼类消化，降低劣质蛋白原料对肠道损伤，抑制鱼肠道细胞凋亡，减少肠道炎症发生，显著改善鱼类肠道屏障的功能。丁酸钠还可用作生长促进剂及免疫刺激剂，在低鱼粉饲料中使用可降低饲料成本，促进鱼类生长发育。因此，丁酸钠是绿色安全可替代抗生素的添加剂，同时植物蛋白适当替代鱼粉可提高经济效益。作者就丁酸钠对肠道消化吸收功能的影响进行重点阐述，以期为鱼类等水产养殖业使用丁酸钠提供科学参考。

关键词: 丁酸钠; 鱼; 肠道; 消化吸收

Abstract: Fish have an imperfect digestive system and low digestive enzyme activity compared with livestock and poultry animals. Plant raw materials often contain anti-nutritional factors and toxic components, resulting in intestinal injury, enteritis, and other diseases. Short-chain fatty acids such as butyric acid can significantly improve fish growth performance, enhance fish health, antioxidant and anti-stress ability. Due to the poor smell of butyric acid affecting the palatability of animals, sodium butyrate, as a substitute for butyric acid, is solid and non-volatile, easy to add and use. Sodium butyrate can promote the development of intestinal epithelial cells, improve the intestinal morphological structure, maintain the integrity of intestinal mucosal barrier, repair the damaged intestinal mucosa, stimulate the synthesis of mRNA and protein in epithelial cells, accelerate the proliferation of intestinal epithelial cells, increase the height of villi, and promote digestion and absorption. Sodium butyrate can destroy the DNA structure of harmful bacteria, and protein can not be synthesized, so that harmful bacteria can not normally divide, inhibit the growth and reproduction of harmful intestinal bacteria, promote the proliferation of beneficial bacteria, maintain the balance of intestinal flora, and significantly improve the diversity of flora. Sodium butyrate can improve fish digestion by stimulating the secretion of digestive enzymes, reduce the intestinal damage caused by poor protein raw materials, inhibit the apoptosis of fish intestinal cells, reduce the occurrence of intestinal inflammation, and significantly improve the function of fish intestinal barrier. Sodium butyrate can be used as a growth promoter and immune stimulant, reduce cost in low fish meal feed and improve fish growth and development. Therefore, sodium butyrate is a green and safe additive that can replace antibiotics, and plant protein can appropriately replace fish meals to improve economic benefits. The author focuses on the effect of sodium butyrate on intestinal digestion and absorption to provide a scientific reference for the application of sodium butyrate in fish and other aquaculture.

Key words: sodium butyrate; fish; intestine; digestion and absorption

中图分类号:

S816.71

李祥, 何金环, 潘春梅, 索江华, 李京. 丁酸钠对鱼类肠道消化吸收的影响研究进展[J]. 中国畜牧兽医, 2022, 49(1): 131-139.

LI Xiang, HE Jinhuan, PAN Chunmei, SUO Jianghua, LI Jing. Research Progress on Effects of Sodium Butyrate on Intestinal Digestion and Absorption of Fish[J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(1): 131-139.

参考文献

[1] TACON A G J,METIAN M.Fish matters:Importance of aquatic foods in human nutrition and global food supply[J].Reviews in Fisheries Science,2013,21(1):22-38.
[2] HOSEINIFAR S H,ANGELES E M,CUESTA A,et al.Prebiotics and fish immune response:A review of current knowledge and future perspectives[J].Reviews in Fisheries Science & Aquaculture,2015,23(4):315-328.
[3] HOSEINIFAR S H,RINGO E,MASOULEH A S,et al.Probiotic,prebiotic and synbiotic supplements in sturgeon aquaculture:A review[J].Reviews in Aquaculture,2016,8(1):89-102.
[4] NAWAZ A,JAVAID A B,IRSHAD S,et al.The functionality of prebiotics as immunostimulant:Evidences from trials on terrestrial and aquatic animals[J].Fish & Shellfish Immunology,2018,76(4):272-278.
[5] 陈业,赵怀宝.丁酸钠在动物肠道功能方面的生物学意义[J].饲料研究,2018,41(2):30-33. CHEN Y,ZHAO H B.Biological significance of sodium butyrate in intestinal function of animals[J].Feed Research,2018,41(2):30-33.(in Chinese)
[6] 左丽君,姜宁,张爱忠.丁酸钠对畜禽机体营养及免疫调控作用的研究进展[J].中国畜牧杂志,2019,55(4):25-28. ZUO L J,JIANG N,ZHANG A Z.Advances in nutrition and immune manipulation action of sodium butyrate on livestock and poultry[J].Chinese Journal of Animal Science,2019,55(4):25-28.(in Chinese)
[7] 李祥,何金环,潘春梅,等.丁酸钠对肉鸡肠道形态与消化吸收功能影响的研究进展[J].中国畜牧兽医,2021,48(5):1603-1612. LI X,HE J H,PAN C M,et al.Research progress of sodium butyrate on intestinal morphology and digestion and absorption function of broilers[J].China Animal Husbandry & Veterinary Medicine,2021,48(5):1603-1612.(in Chinese)
[8] 胡宁敏,陈代文,余冰,等.丁酸对断奶仔猪腹泻的缓解作用及其机理研究进展[J].动物营养学报,2020,32(5):1973-1979. HU N M,CHEN D W,YU B,et al.Research progress on relaxation effects of butyric acid on diarrhea in weaned piglets and its mechanism[J].Chinese Journal of Animal Nutrition,2020,32(5):1973-1979.(in Chinese)
[9] 李海英,张俐华,翟卫爽,等.丁酸钠替代饲用抗生素对白羽肉仔鸡生产性能和免疫器官指数的影响[J].新疆农业科学,2014,51(4):755-760. LI H Y,ZHANG L H,ZHAI W S,et al.Effect of sodium butyrate used as substitute for antibiotics feed additive on production performance and immune organ index of White broilers[J].Xinjiang Agricultural Sciences,2014,51(4):755-760.(in Chinese)
[10] LIN F,LI X,WEN J,et al.Effects of coated sodium butyrate on performance,diarrhea,intestinal microflora and barrier function of pigs during the first 2-week post-weaning[J].Animal Feed Science and Technology,2020,263(5):114464.
[11] 芦娜,王恬,钟翔.不同的丁酸产品在猪禽生产中的应用研究进展[J].动物营养学报,2015,27(2):359-368. LU N,WANG T,ZHONG X.Recent advances on application of different butyrate products in pigs and poultry production[J].Chinese Journal of Animal Nutrition,2015,27(2):359-368.(in Chinese)
[12] 吴东霖,徐萍,邵凯,等.丁酸在幼龄反刍动物中的作用机理及其产品应用的研究进展[J].动物营养学报,2020,32(3):1034-1046. WU D L,XU P,SHAO K,et al.Mechanism of butyric acid and application of its products in young ruminants[J].Chinese Journal of Animal Nutrition,2020,32(3):1034-1046.(in Chinese)
[13] 左丽君,陈想,王可鑫,等.丁酸钠对断奶羔羊胃肠道发育的影响[J].动物营养学报,2020,32(4):1916-1926. ZUO L J,CHEN X,WANG K X,et al.Effects of sodium butyrate on gastrointestinal tract development of weaned lambs[J].Chinese Journal of Animal Nutrition,2020,32(4):1916-1926.(in Chinese)
[14] LIU W,YANG Y,ZHANG J,et al.Effects of dietary microencapsulated sodium butyrate on growth,intestinal mucosal morphology,immune response and adhesive bacteria in juvenile common carp (Cyprinus carpio) pre-fed with or without oxidised oil[J].British Journal of Nutrition,2014,112(1):15-29.
[15] DA SILVA B C,VIEIRA F D N,PEDREIRA M J L,et al.Butyrate and propionate improve the growth performance of Litopenaeus vannamei[J].Aquaculture Research,2016,47(2):612-623.
[16] ESTENSORO I,BALLESTER-LOZANO G,BENEDITO-PALOS L,et al.Dietary butyrate helps to restore the intestinal status of a marine teleost (Sparus aurata) fed extreme diets low in fish meal and fish oil[J].PLoS One,2016,11(11):e0166564.
[17] SAFARI R,HOSEINIFAR S H,KAVANDI M.Modulation of antioxidant defense and immune response in zebra fish (Danio rerio) using dietary sodium propionate[J].Fish Physiology and Biochemistry,2016,42(6):1733-1739.
[18] ABDEL-TAWWAB M,KHATTABY A,MONIER M.Dietary acidifiers blend enhanced the production of Nile tilapia (Oreochromis niloticus),striped mullet (Mugil cephalus),and African catfish (Clarias gariepinus) polycultured in earthen ponds[J].Aquaculture International,2019,27(2):369-379.
[19] HOSEINIFAR S H,SUN Y Z,CAIPANG C M.Short-chain fatty acids as feed supplements for sustainable aquaculture:An updated view[J].Aquaculture Research,2017,48(4):1380-1391.
[20] KIHARA M,SAKATA T.Influences of incubation temperature and various saccharides on the production of organic acids and gases by gut microbes of rainbow trout Oncorhynchus mykiss in a micro-scale batch culture[J].Journal of Comparative Physiology B-Biochemical Systemic and Environmental Physiology,2001,6:441-447.
[21] BEDFORD A,GONG J.Implications of butyrate and its derivatives for gut health and animal production[J].Animal Nutrition,2018,4(2):151-159.
[22] 曲悦.丁酸钠对动物胃肠道影响的研究进展[J].中国畜牧兽医,2009,36(8):23-26. QU Y.Research advance of sodium butyrate on the impact of the animal gastrointestinal tract[J].China Animal Husbandry & Veterinary Medicine,2009,36(8):23-26.(in Chinese)
[23] 庄金秋,梅建国,姚春阳,等.丁酸钠及其在断奶仔猪腹泻中的应用研究进展[J].猪业科学,2015,32(6):94-96. ZHUANG J Q,MEI J G,YAO C Y,et al.Research progress of sodium butyrate and its application in diarrhea of weaned piglets[J].Swine Industry Science, 2015,32(6):94-96.(in Chinese)
[24] PIVA A,ANFOSSI P,MEOLA E,et al.Effect of microcapsulation on absorption processes in the pig[J].Livestock Production Science,1997,51(1-3):53-61.
[25] PIVA A,GRILLI E,FABBRI L,et al.Free versus microencapsulated organic acids in medicated or not medicated diet for piglets[J].Livestock Science,2007,108(1-3):214-217.
[26] 张美玲,单承杰,杜震宇.益生菌与鱼类肠道健康研究进展[J].水产学报,2021,45(1):147-157. ZHANG M L,SHAN C J,DU Z Y.Research advances on probiotics and fish gut health[J].Journal of Fisheries of China,2021,45(1):147-157.(in Chinese)
[27] 米海峰,孙瑞健,张璐,等.鱼类肠道健康研究进展[J].中国饲料,2015,26(15):19-22. MI H F,SUN R J,ZHANG L,et al.Review on the intestinal health of fish[J].China Feed,2015,26(15):19-22.(in Chinese)
[28] TIMBERMONT L,LANCKRIET A,DEWULF J,et al.Control of clostridium perfringens-induced necrotic enteritis in broilers by target-released butyric acid,fatty acids and essential oils[J].Avian Pathology,2010,39(2):117-121.
[29] VINOLO M A,RODRIGUES H G,NACHBAR R T,et al.Regulation of inflammation by short chain fatty acids[J].Nutrients,2011,3(10):858-876.
[30] LIU J,LUMPKINS B,MATHIS G,et al.Evaluation of encapsulated sodium butyrate with varying releasing times on growth performance and necrotic enteritis mitigation in broilers[J].Poultry Science,2019,98(8):3240-3245.
[31] ABDEL-MOHSEN H H,WASSEF E A,EL-BERMAWY N M,et al.Advantageous effects of dietary butyrate on growth,immunity response,intestinal microbiota and histomorphology of European seabass (Dicentrarchus labrax) fry[J].Egyptian Journal of Aquatic Biology and Fisheries,2018,22(4):93-110.
[32] ZHOU J S,GUO P,YU H B,et al.Growth performance,lipid metabolism,and health status of grass carp (Ctenopharyngodon idella) fed three different forms of sodium butyrate[J].Fish Physiology and Biochemistry,2019,45(1):287-298.
[33] WU P,TIAN L,ZHOU X Q,et al.Sodium butyrate enhanced physical barrier function referring to Nrf2,JNK and MLCK signaling pathways in the intestine of young grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2018,73(8):121-132.
[34] DAWOOD M A O,EWEEDAH N M,ELBIALY Z I,et al.Dietary sodium butyrate ameliorated the blood stress biomarkers,heat shock proteins,and immune response of Nile tilapia (Oreochromis niloticus) exposed to heat stress[J].Journal of Thermal Biology,2020,88:102500
[35] 王经远,戈贤平,周群兰,等.发酵豆粕替代复合植物蛋白质源对团头鲂幼鱼生长性能及肠道健康的影响[J].动物营养学报,2019,31(11):5111-5121. WANG J Y,GE X P,ZHOU Q L,et al.Effects of compound plant protein sources replaced by fermented soybean meal on growth performance and intestinal health of juvenile blunt snout bream(Megalobrama amblycephala)[J].Chinese Journal of Animal Nutrition,2019,31(11):5111-5121.(in Chinese)
[36] 郑瑞耕.丁酸钠对淡水鱼类生长性能及肠粘膜结构的影响[D].福州:福建农林大学,2008. ZHENG R G.The effect of sodium butyrate on the growth performance and intestinal mucous structure of fresh water fish[D].Fuzhou:Fujian Agriculture And Forestry University,2008.(in Chinese)
[37] 张俊智.饲料中添加丁酸钠对黄鳝生长、免疫及肠道健康的影响[D].长沙:湖南农业大学,2015. ZHANG J Z.Effect of dietary sodium butyrate on growth,immune and intestinal health in rice field eel(Monopterus albus)[D].Changsha:Hunan Agricultural University,2015.(in Chinese)
[38] RIMOLDI S,FINZI G,CECCOTTI C,et al.Butyrate and taurine exert a mitigating effect on the inflamed distal intestine of European sea bass fed with a high percentage of soybean meal[J].Fisheries and Aquatic Sciences,2016,19(40):1-14.
[39] LUZ J R,SOUZA R A P,BIBIANO M J F,et al.Use of sodium butyrate in the feeding of Arapaima gigas (Schinz,1822) juvenile[J].Aquaculture,2019,510:248-255.
[40] 宋增福,吴天星.鱼类肠道正常菌群研究进展[J].水产科学,2007,27(8):471-474. SONG Z F,WU T X.Review on intestinal normal microflora in fish[J].Fisheries Science,2007,27(8):471-474.(in Chinese)
[41] PIAZZON M C,CALDUCH-GINER J A,FOUZ B,et al.Under control:How a dietary additive can restore the gut microbiome and proteomic profile,and improve disease resilience in a marine teleostean fish fed vegetable diets[J].Microbiome,2017,5(1):164.
[42] TIAN L,ZHOU X Q,JIANG W D,et al.Sodium butyrate improved intestinal immune function associated with NF-kappa B and p38MAPK signalling pathways in young grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2017,66(40):548-563.
[43] RIMOLDI S,GLIOZHENI E,ASCIONE C,et al.Effect of a specific composition of short- and medium-chain fatty acid 1-monoglycerides on growth performances and gut microbiota of gilthead sea bream (Sparus aurata)[J].Peer J,2018,6:5355.
[44] HOSEINIFAR S H,DADAR M,RINGO E.Modulation of nutrient digestibility and digestive enzyme activities in aquatic animals:The functional feed additives scenario[J].Aquaculture Research,2017,48(8):3987-4000.
[45] AALAMIFAR H,SOLTANIAN S,VAZIRZADEH A,et al.Dietary butyric acid improved growth,digestive enzyme activities and humoral immune parameters in Barramundi (Lates calcarifer)[J].Aquaculture Nutrition,2020,26(1):156-164.
[46] ZHOU C,LIN H,HUANG Z,et al.Effect of dietary sodium butyrate on growth performance,enzyme activities and intestinal proliferation-related gene expression of juvenile golden pompano Trachinotus ovatus[J].Aquaculture Nutrition,2019,25(6):1261-1271.
[47] 谢一荣,吴锐全.鱼类消化酶研究及其在水产养殖中的应用[J].广东饲料,2005,14(2):15-18. XIE Y R,WU R Q.Study on fish digestive enzymes and its application in aquaculture[J].Guangdong Feed,2005,14(2):15-18.(in Chinese)
[48] 宋霖,蔡春芳,叶元土,等.四种植物蛋白对黄颡鱼肠道形态结构的影响[J].淡水渔业,2012,42(6):54-60. SONG L,CAI C F,YE Y T,et al.Effects of four kinds of plant protein on intestinal morphology of Pelteobagrus fulvidraco[J].Freshwater Fisheries,2012,42(6):54-60.(in Chinese)
[49] 韩庆炜,梁萌青,姚宏波,等.鲈鱼对7种饲料原料的表观消化率及其对肝脏、肠道组织结构的影响[J].渔业科学进展,2011,32(1):32-39. HAN Q W,LIANG M Q,YAO H B,et al.Effects of seven feed ingredients on growth performance,and liver and intestine histology of Lateolabrax japonicas[J].Progress in Fishery Sciences,2011,32(1):32-39.(in Chinese)
[50] 刘艳莉,胡毅,钟蕾,等.丁酸对动物肠道健康的影响及水产应用前景[J].水产科学,2019,38(2):276-281. LIU Y L,HU Y,ZHONG L,et al.A review:Research progress on effects of butyric acid on intestinal health in animals and its application in aquaculture[J].Fisheries Science,2019,38(2):276-281.(in Chinese)
[51] NIKLASSON L,SUNDH H,FRIDELL F,et al.Disturbance of the intestinal mucosal immune system of farmed Atlantic salmon (Salmo salar),in response to long-term hypoxic conditions[J].Fish & Shellfish Immunology,2011,31(6):1072-1080.
[52] KIENER T K,SLEPTSOVA-FRIEDRICH I,HUNZIKER W.Identification,tissue distribution and developmental expression of TJP1/ZO-1,TJP2/ZO-2 and TJP3/ZO-3 in the zebrafish,Danio rerio[J].Gene Expression Patterns,2007,7(7):767-776.
[53] XU F,YE Y T,CAI C F,et al.Observation of the middle intestinal tight junction structure,cloning and studying tissue distribution of the four Claudin genes of the grass carp (Ctenopharyngodon idellus)[J].Fish Physiology and Biochemistry,2014,40(6):1783-1792.
[54] BAKKE-MCKELLEP A M,PENN M H,SALAS P M,et al.Effects of dietary soyabean meal,inulin and oxytetracycline on intestinal microbiota and epithelial cell stress,apoptosis and proliferation in the teleost Atlantic salmon (Salmo salar L.)[J].British Journal of Nutrition,2007,97(4):699-713.
[55] LAURICELLA M,EMANUELE S,D'ANNEO A,et al.JNK and AP-1 mediate apoptosis induced by bortezomib in HepG2 cells via FasL/Caspase-8 and mitochondria-dependent pathways[J].Apoptosis,2006,11(4):607-625.
[56] LIU Y,CHEN Z,DAI J,et al.Sodium butyrate supplementation in high-soybean meal diets for turbot (Scophthalmus maximus L.):Effects on inflammatory status,mucosal barriers and microbiota in the intestine[J].Fish & Shellfish Immunology,2019,88:65-75.
[57] SONG X,ZHAO J,BO Y,et al.Aeromonas hydrophila induces intestinal inflammation in grass carp (Ctenopharyngodon idella):An experimental model[J].Aquaculture,2014,434:171-178.
[58] BAEVERFJORD G,KROGDAHL Å.Development and regression of soybean meal induced enteritis in Atlantic salmon,Salmo salar L.,distal intestine:A comparison with the intestines of fasted fish[J].Journal of Fish Diseases,1996,19(5):375-387.
[59] MEIJER K,DE VOS P,PRIEBE M G.Butyrate and other short-chain fatty acids as modulators of immunity:What relevance for health?[J].Current Opinion in Clinical Nutrition & Metabolic Care,2010,13(6):715-721.
[60] SEGAIN J,DE LA BLÉTIERE D R,BOURREILLE A,et al.Butyrate inhibits inflammatory responses through NF-κB inhibition:Implications for Crohn's disease[J].Gut,2000,47(3):397-403.
[61] 张淞琳,常建波,叶继丹,等.丁酸钠对美洲鳗鲡摄食、生长性能和抗氧化能力的影响[J].福建农业学报,2011,26(4):549-551. ZHANG S L,CHANG J P,YE J D,et al.Effects of sodium butyrate on feeding,growth performance and antioxidant capacity of Anguilla rostrata[J].Fujian Journal of Agricultural Sciences,2011,26(4):549-551.(in Chinese)
[62] 孙浪,刘臻,郝光,等.丁酸钠对鲫鱼生长和肠细胞增殖的影响[J].中国水产科学,2013,20(4):893-901. SUN L,LIU Z,HAO G,et al.Effects of sodium butyrate on growth and intestinal cell proliferation of Carassius auratus[J].Journal of Fishery Sciences of China,2013,20(4):893-901.(in Chinese)
[63] ROBLES R,LOZANO A B,SEVILLA A,et al.Effect of partially protected butyrate used as feed additive on growth and intestinal metabolism in sea bream (Sparus aurata)[J].Fish Physiology and Biochemistry,2013,39(6):1567-1580.
[64] ZHANG J,ZHONG L,CHI S,et al.Sodium butyrate supplementation in high-soybean meal diets for juvenile rice field eel (Monopterus albus):Effects on growth,immune response and intestinal health[J].Aquaculture,2020,520:734952.
[65] 唐建洲,曹申平,瞿符发,等.纳米缓释丁酸钠对草鱼生长性能、血清生化指标、肠道黏膜形态及PepT1基因表达的影响[J].水生生物学报,2021,45(4):764-773. TANG J Z,CAO S P,QU F F,et al.Effects of sustained release nanosphere sodium butvrate on the growth performance,serum biochemical indices,intestinal mucosal morphology and PepT1 mRNA expression in intestinal of grass carp (Ctenopharyngodon idellus)[J].Acta Hydrobiologica Sinica,2021,45(4):764-773.(in Chinese)
[66] 王海瑞,胡俊茹,赵红霞,等.饲料中添加丁酸钠对黄颡鱼幼鱼非特异性免疫、抗氧化指标及肠道黏膜形态的影响[J].动物营养学报,2021,33(9):5379-5390. WANG H R,HU J R,ZHAO H X,et al.Effects of dietary sodium butyrate on non-specific immune,antioxidant indices and intestinal mucosal morphology of juvenile yellow catfish (Pelteobagrus fulvidraco)[J].Chinese Journal of Animal Nutrition,2021,33(9):5379-5390.(in Chinese)
[67] WU X,WANG L,XIE Q,et al.Effects of dietary sodium butyrate on growth,diet conversion,body chemical compositions and distal intestinal health in yellow drum(Nibea albiflora,Richardson)[J].Aquaculture Research,2020,51(1):69-79.
[68] 魏朝青,周慧慧,王旋,等.高植物蛋白质饲料中添加丁酸钠对大菱鲆幼鱼生长性能、营养物质表观消化率及肝脏抗氧化功能的影响[J].动物营养学报,2017,29(9):3392-3402. WEI C Q,ZHOU H H,WANG X,et al.Effects of adding different levels of sodium butyrate in high plant protein diets on growth performance,nutrient apparent digestibility coefficients and liver antioxidant function of juvenile turbot(Scophthalmus maximus L.)[J].Chinese Journal of Animal Nutrition,2017,29(9):3392-3402.(in Chinese)
[69] 张含乐,解绶启,贠彪,等.低鱼粉饲料中添加丁酸钠对黄颡鱼生长性能、饲料利用和肠道组织结构的影响[J].中国饲料,2021,22(1):96-101. ZHANG H L,XIE S Q,YUAN B,et al.Effects of sodium butyrate on the growth performance,feed utilization and intestinal morphology of Yellow catfish (Pelteobagrus fulvidraco) fed low fish meal diet[J].China Feed,2021,22(1):96-101.(in Chinese)