›› 2012, Vol. 39 ›› Issue (4): 39-41.

• 生物技术 • 上一篇    下一篇

褪黑激素受体1a基因在内蒙古绒山羊垂体中的表达

郑竹清1,2, 石玲1,2, 王梦一3, 吴敬4, 刘志红1,2, 张燕军1,2, 苏蕊1,2, 赵艳红1,2   

  1. 1. 内蒙古农业大学动物科学学院,内蒙古呼和浩特 010018;2. 内蒙古自治区动物遗传育种与繁殖重点实验室,内蒙古呼和浩特 010018;3. 呼伦贝尔市环境科学研究所,内蒙古海拉尔 021008;4. 内蒙古农业大学食品工程与科学学院,内蒙古呼和浩特 010018
  • 收稿日期:2011-10-24 修回日期:1900-01-01 出版日期:2012-04-20 发布日期:2012-04-20
  • 通讯作者: 赵艳红

The Expression of MTNR1a Gene in Pituitary of Inner Mongolia Cashmere Goats

ZHENG Zhu-qing1,2, SHI Ling1,2, WANG Meng-yi3, WU Jing4, LIU Zhi-hong1,2, ZHANG Yan-jun1,2, SU Rui1,2, ZHAO Yan-hong1,2   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China;2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction,Huhhot 010018, China;3. Hulunbeier Institute of Environmental Science, Hailaer 021008,China;4. College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018,China
  • Received:2011-10-24 Revised:1900-01-01 Online:2012-04-20 Published:2012-04-20

摘要： 本试验从内蒙古绒山羊脑垂体中提取总RNA,根据已发表的相近物种褪黑激素受体1a(melatonin receptor 1 a,MTNR1a)基因序列设计引物,利用实时荧光定量逆转录多聚酶链反应(real-time quantitative,RT-PCR)技术、组织原位杂交技术检测MTNR1a基因在内蒙古绒山羊垂体中的表达。结果显示,通过RT-PCR方法检测到MTNR1a基因在内蒙古绒山羊垂体中表达,利用原位杂交技术进一步确定MTNR1a基因在垂体中表达分布。结果提示,垂体是褪黑激素作用的靶器官,褪黑激素对绒山羊季节性繁殖的影响有可能是与垂体分泌的某些激素相互作用而发挥作用的。

关键词: MTNR1a; 内蒙古绒山羊; RT-PCR; 原位杂交; 垂体; 表达

Abstract: In the study , the total RNA was extracted from pituitary of Inner Mongolia cashmere goats. The primers were designed according to the published MTNR1a in similar species . The expression of melatonin receptor gene MTNR1a was detected by using RT-PCR and in situ hybridization in Inner Mongolia cashmere goats . The results showed that the expression of MTNR1a was successfully detected by using RT-PCR ,by using in situ hybridization futher defined the expression and distribution of MTNR1a. The results suggested that the pituitary is the target organ of the melatonin ,the hormone secreted by pituitary may play an important role in the effect of melatonin on seasonal breeding of cashmere goats.

Key words: MTNR1a; Inner Mongolia cashmere goats; RT-PCR; in situ hybridization; pituitary; expression

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