Abstract: A pair of primers was designed and synthesized according to the bovine Hepcidin gene sequence in GenBank. Coding sequence of Hepcidin gene was amplified by RT-PCR technology from total RNA of yak’s live, the sequence of Hepcidin was analysed and phylogenetic tree of Hepcidin protein was constructed. The results showed that a 322 bp cDNA sequence of Hepcidin gene was cloned, including 289 bp open reading frames, encoding 82 amino acids which contained 22 amino acids coded signal peptide. Forecast for protein molecular weight and isoelectric points, was 8.88 ku and 9.24 respectively. This gene region had high conservation in the bovine species, compared with the ordinary cow, the homology of Hepcidin sequence was up to 99 percent.The phylogenetic tree conformed to the laws of species evolution. The research laid an important foundation on the full-length cDNA gene cloning and study.

Key words: yak; Hepcidin gene; clone; sequence analysis