Abstract: 96 matrix antibiotic susceptibility testing kit was designed based on agar dilution method. According to National Committee for Clinical Laboratory Standards and the kid, susceptibility of 98 E.coli strains isolated from raisers in the main large scale farms, students of kindergarten and middle school to Enrofloxacin, Ciprofloxacin, Norfloxacin and Sanhuangtang. The results showed that the MBC and MIC was high symmetry of two methods. The average of difference was in one dilution range respectively and the coincident ratio all reached 90% in 0.5 μg/mL difference range. The range of relativity was 0.94 to 0.99 of MBC and MIC. We found that the parallelism of MBC and MIC was high from the index. The results of the two methods were closely compared to the total frequency of susceptibility, the coincident ratio was 93.93%. 96 matrix antibiotic susceptibility testing kit can determine MIC of Sanhuangtang exactly ranged from 125 to 500 mg/mL. We can conclude that 96 matrix antibiotic susceptibility testing kit is a new kit which can adapt to large scale lab antibiotic susceptibility determination that simple, exact and fast, large flux, economize, which can test 96 bacteria for one antibiotic or Chinese traditional medicine combined with 96 holes incubated board one time.

Key words: 96 matrix antibiotic susceptibility testing kit; micro dilution method; E.coli; large flux