千里光超微粉的镇痛机制研究

doi:10.16431/j.cnki.1671-7236.2019.08.034

Abstract

Abstract:

To investigate the analgesic mechanism of Senecio scandens ultrafine powder, and provide theoretical basis and reference for the S.scandens analgesic.In this study, 6-week-old Kunming mice were used as experimental animals.The suspension of S.scandens ultrafine powder was prepared, and S.scandens ultrafine powder was administered at high (360 mg/kg·BW), medium (180 mg/kg·BW) and low (90 mg/kg·BW) dose groups, after 7 days of continuous administration by gavage, follow-up related experiments were carried out.The analgesic effect was verified by formaldehyde-induced pain model and hot-water tail-flick, and it was used to distinguish central analgesia and peripheral analgesia.The analgesic mechanism of S.scandens ultrafine powder was investigated by naloxone antagonistic test, reserpine antagonistic test, and detecting the concentrations of serotonin (5-HT) and nitric oxide (NO) in serum and brain tissue.The experiment proved that S.scandens ultrafine powder could increase the pain threshold of formaldehyde-induced pain model and hot-water tail-flick in a dose-dependent manner;Naloxone and reserpine as opioid receptor blockers and anti-noradrenergic nerve terminal, respectively, could partially antagonize the analgesia effect of S.scandens ultrafine powder.S.scandens ultrafine powder could significantly increase the content of 5-HT in brain tissue and reduce the content of 5-HT in serum (P<0.05), but was not significant for NO.S.scandens ultrafine powder had central and peripheral analgesic effects in a dose-dependent manner.The analgesic pathway was related to opioid receptors and NE;It also exerted its analgesic effect by increasing the contents of central NO and 5-HT, and decreasing the contents of peripheral NO and 5-HT, but the effect of regulating NO content was not obvious.

Key words: S.scandens ultrafine powder; central analgesia; peripheral analgesia

CLC Number:

S853.74

ZHOU Yong, WEN Jiaying, LIN Qisheng, HONG Yuqi, LIANG Yinping, ZHANG Qing, LIN Hongying, CHEN Jinjun. Study on Analgesic Mechanism of Senecio scandens Ultrafine Powder[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(8): 2470-2477.

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Study on Analgesic Mechanism of Senecio scandens Ultrafine Powder

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