Abstract: This study used Real-time PCR to detect the changes of expression quantity of claudin 3 (CLDN-3), cytokeratin 8 (KRT8) and mucin 1 (MUC1) three proteins mRNA in IPEC-J2 cells at three different time points.IPEC-J2 cells were infected by TGEV, we explored whether Lactobacillus amylovorus metabolites could enhance cell barrier function by maintaining or improving mRNA expression of CLDN-3, KRT8 and MUC1, whether had effects on the barrier function of IPEC-J2 infected by TGEV.The results showed that mRNA expressions of MUC1, CLDN-3 and KRT8 in the normal cell control group did not change significantly with time (P> 0.05); MRS medium culture group did not change significantly compared to the normal cell control group(P> 0.05); Gene expressions of MUC1, CLDN-3 and KRT8 proteins in Lactobacillus amylovorus metabolites alone treatment group showed significant time-dependent effect (P< 0.05).After being treated 24, 36 and 48 h, gene expressions of MUC1, CLDN-3 and KRT8 proteins in Lactobacillus amylovorus metabolites+TGEV experiment group was significantly higher than virus control group (P< 0.05).Lactobacillus amylovorus metabolites could improve MUC1, CLDN-3 and KRT8 expressions in IPEC-J2 cells, while being able to negatively regulate MUC1, CLDN-3 and KRT8 expressions that TGEV induced in IPEC-J2 cells, thereby inhencing the barrier function of IPEC-J2 cell against TGEV.

Key words: Lactobacillus amylovorus; TGEV; IPEC-J2