猪传染性胃肠炎病毒和猪流行性腹泻病毒二重PCR检测方法的建立

doi:10.16431/j.cnki.1671-7236.2015.06.008

Abstract

Abstract: Two pairs of primers used to respectively amplify transmissible gastroenteritis virus (TGEV) S gene and porcine epidemic diarrhea virus (PEDV) M gene were designed to develop a method of differential diagnosis of TGEV and PEDV.The established double PCR could detect S gene of TGEV with the length of 299 bp and M gene of PEDV with the length of 437 bp.Negative results using CSFV,PCV2,PRRSV and PRV as control were obtained.The detection limit of this method was 10⁴ copies/μL.68 clinical samples collected from swine farm were submitted to detect TGEV and PEDV,and the result showed that the established double PCR method with the characteristics of high sensitivity and high specificity could be widely used in clinical diagnosis and epidemiological investigation.

Key words: transmissible gastroenteritis virus (TGEV); porcine epidemic diarrhea virus (PEDV); double PCR; diagnosis

CLC Number:

LU Chao, ZHANG Li, WANG Li-li, YANG Chun-lei, HAN Wei. Establishment of Double PCR Detection Method of Transmissible Gastroenteritis Virus (TGEV) and Porcine Epidemic Diarrhea Virus (PEDV)[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(6): 1377-1382.

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Establishment of Double PCR Detection Method of Transmissible Gastroenteritis Virus (TGEV) and Porcine Epidemic Diarrhea Virus (PEDV)

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