牦牛乳房炎病原无乳链球菌的分离鉴定与耐药性分析

doi:10.11843/j.issn.1671-7236.2015.03.034

Abstract

Abstract: In the experiment,we aseptically collected yak milk in Tibet,and then separated and purified bacteria,and identified by PCR; At the same time,researched the drug sensitivity of separated strains to provide reference for the prevention and curement of the pathogen in production practice.The results showed that the purified bacteria were gram positive Streptococcus and identified as Streptococcus agalactiae through biochemical identification and PCR.Drug sensitivity test showed that the separated strains were sensitive to most of penicillins,aminoglycosides and chloramphenicols.The research provided the reference for the prevention and curement of mastitis caused by yak Streptococcus agalactiae in Tibet.

Key words: yak; mastitis; Streptococcus agalactiae; identification; drug resistance

CLC Number:

S852.61

LIU Fu-yin, JIANG Pei-pei, GENG Ya-ya, CHE Chen, YUE Lei, HAO Bao-qing. Isolation,Identification and Drug Resistance Analysis of Yak Mastitis Pathogen Streptococcus agalactiae[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(3): 728-733.

References

[1] 牛春娥,张利平,孙俊峰,等.我国牦牛资源现状及其产品开发利用前景分析[J].安徽农业科学,2009,37(17):8002-8005.

[2] 李海梅,何胜华,刘天一,等.牦牛乳物理化学特性的研究进展[J].中国乳品工业,2009,37(8):35-40.

[3] 孙西峰,沈廷银.牦牛隐性乳房炎发病情况的调查[J].青海畜牧兽医杂志.2009,39(2):10.

[4] Buchanan R E.伯杰细菌鉴定手册[M].北京:北京农业大学出版社,1991.

[5] Radhan P,Gopinath S M,Reddy G R,et al.Detection of major pathogens in bovine subclinical mastitis by multiplex PCR directly from milk samples in presence of an internal control[J].Indian Journal of Fundamental and Applied Life Sciences,2011,1(4):208-209.

[6] 田淑琴,邓孝廷,陈朝喜.兽医药理学实验指导[M].北京:中国农业大学出版社,2010.

[7] Gilchrist T L,Smith D G E,Fitzpatrick J L,et al.Comparative molecular analysis of ovien and bovine Streptococcus uberis isolates[J].J Dairy Sci,2013,96(2):962-970.

[8] Fernandez-Garayzabal J F,Fernandez E,Las Heras A,et al.Streptococcus parasanguinis: New pathogen associated with asymptomatic mastitis in sheep[J].Emerging Infections Diseases,1998,4(4):645-647.

[9] Watkins G H,Burriel A R,Jones J E T.A field investigation of subclinical mastitis in sheep in Southern England[J].British Veterinary Journal,1991,147(5):413-420.

[10] 田珂,贾爱卿,陈善真,等.罗非鱼无乳链球菌生物学特性与致病力研究[J].中国畜牧兽医,2013,40(6):203-206.

[11] Schuchat A.Group BStreptococcus[J].Lancet,1999,353:51-55.

[12] Zhang J H,Yuan L,Yang Y H.Perinatal colonization of group B Streptococcus: A study of 600 cases in Beijing Tiantan hospital[J].China J Epidemiol,1995,16:36-38.

[13] 冯现维.乳房链球菌对克林霉素耐药基因的定位[J].中国预防兽医学报,2001,23(2):134-136.

[14] 李宏胜,郁杰,罗金印,等.牛源性无乳链球菌血清型分析及抗生素耐药性研究[J].中国畜牧兽医,2012,39(1):164-167.

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Isolation,Identification and Drug Resistance Analysis of Yak Mastitis Pathogen Streptococcus agalactiae

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