利巴韦林对猫细小病毒感染诱导F81猫肾细胞中凋亡相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2015.02.011

Abstract

Abstract: The F81 cells were divided into three groups,control group,feline panleukopenia virus (FPV) infected group and ribavirin treatment group,we used Real-time fluorescent quanlitative PCR to detect the expression of Bcl-2,Bcl-xl,Bax and Caspase 3.Real-time fluorescent quanlitative PCR results showed that the number of apoptotic cells increased in FPV infected group compared with the control group,and mRNA levels of Bcl-2 was inhibited,which was an anti-apoptotic gene,while the expressions of Bax and Caspase 3 were both increased.In ribavirin treatment group,expression levels of Bcl-2 and Bcl-xl were significantly higher than that of FPV infected group (P<0.05),and had a peak at 48 h in all time points.However,mRNA levels of Bax and Caspase 3 in ribavirin treatment group were significantly lower than that of FPV infected group (P<0.05),and the lowest levels were at 72 h in all time points.In a word,ribavirin could inhibit the apoptosis of F81 cells infected by FPV at the genetic level.

Key words: ribavirin; F81 cells; gene expression; cell apoptosis

CLC Number:

Q786

HU Lian-mei, ZHENG Liang-yan, YU Wen-lan, LI Ya-feng, TANG Zhao-xin. Effects of Ribavirin on Expression of Apoptotic Related Genes of F81 Cells Infected by Feline Panleukopenia Virus[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(2): 309-316.

References

[1] Parker W B.Metabolism and antiviral activity of ribavirin[J].Virus Res,2005,107(2):165-171.
[2] Kamar N,Rostaing L,Abravanel F,et al.Ribavirin therapy inhibits viral replication on patients with chronic hepatitise virus infection[J].Gastroenterology,2010,139(5):1612-1618.
[3] 张燕,朱贞,祝双利,等.利巴韦林注射液在细胞水平上抑制EV71病毒复制的初步研究[J].中华实验和临床病毒学杂志,2009,23(1):44-46.
[4] 赵海忠,靳波,周祖涛,等.病毒唑超声雾化疗法治疗犬支气管炎疗效观察[J].中国畜牧兽医,2008,35(3):118-119.
[5] 房世雄,杨林.病毒唑治疗犬细小病毒病[J].中国兽医杂志,1996,22(3):40-41.
[6] 盛晓蓉,费志洁,吴亦伦.干扰素、病毒唑抗呼吸道合胞病毒的体外观察[J].中国临床药理学与治疗学,2001,6(2):114-116.
[7] Yamazaki J,Hasebe N,Nagafuchi S,et al.Expression of apoptosis-related gene mRNAs in feline T-cells infected with feline immunodeficiency virus (FIV)[J].Vet Microbiol,2004,101(1):1-8.
[8] Gerber H P,Dixit V,Ferrara N.Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl-2 and A1 in vascular endothelial cells[J].J Biol Chem,1998,273(21):13313-13316.
[9] Sano J,Nagafuchi S,Yamazaki J,et al.Effect of antineoplastic drugs on the expression of Bcl-2 and Bcl-xl genes in the feline T-cell leukemia cell line[J]. Res Vet Sci,2005,79(3):197-201.
[10] Hetz C.Bcl-2 protein family.Essential regulators of cell death.Preface[J].Adv Exp Med Biol,2010,687:Ⅶ-Ⅷ.
[11] 弓娟琴,陈志强,李文忠,等.Fas介导的凋亡与Caspase家族[J].国外医学·皮肤性病学分册,2001,27(5):279-281.
[12] Takahashi K,Ohyabu Y,Schaffer S W,et al.Cellular characterization of an in vitro cell culture model of seal-induced cardiac ischaemia[J].J Pharm Pharmacol,2001,53(3):379-386.

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Effects of Ribavirin on Expression of Apoptotic Related Genes of F81 Cells Infected by Feline Panleukopenia Virus

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