Abstract: 【Objective】 The extraction process of dandelion (general goods) extract was optimized,and its anti-inflammatory,antibacterial and growth-promoting effect were explored. 【Method】 High performance liquid chromatography (HPLC) was used to determine the total content of index components (caffeic acid and chichoric acid) in dandelion extract.Based on the ratio of solid-liquid,the number of extractions,and the extraction time,and the total content of index components in the extract and the extract yield as the evaluation indexes,the optimal extraction process was determinded by orthogonal experiments.Through the xylene-induced mouse ear swelling model,the effects of different doses of dandelion extract on the degree of ear swelling and the inhibition rate of ear swelling in mice were explored,and its anti-inflammatory effect was evaluated.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of dandelion extract against Staphylococcus aureus and Escherichia coli were determined by microbroth dilution method,and its antibacterial effect was evaluated.The mice were given dandelion extract continuously for 20 days,and the body weight and feed intake of mice were recorded,and the average daily gain and average daily feed intake of mice were calculated to evaluate its growth-promoting effect. 【Result】 The optimal extraction process for dandelion extract was a solid-liquid ratio of 1∶12,reflux extraction twice for 2 hours each time.Under these extraction conditions,the average total content of index components and the average yield of dandelion extract were 9.83 mg/g and 33.61%,respectively.The results of the anti-inflammatory test showed that,the inhibition rate of ear swelling in medium- and high-dose groups of dandelion extract was 45.1% and 51.2%,respectively.The results of antibacterial test showed that the MIC of dandelion extract against Staphylococcus aureus and Escherichia coli were 15.63 and 125 mg/mL,respectively,and the MBC was 250 and 125 mg/mL,respectively.In the growth promotion test,compared with blank group,the average daily weight gain of mice in medium-dose group was significantly increased (P＜0.05),and the average daily feed intake and feed-to-weight ratio were significantly decreased (P＜0.05). 【Conclusion】 The optimal extraction process conditions for dandelion extract were a solid-liquid ratio of 1∶12,extraction twice,each time for 2 hours.Under these process conditions,the total content of index components and the yield of dandelion extract prepared were relatively high,and it also had certain antibacterial,anti-inflammatory and growth-promoting effects.

Key words: general goods; dandelion; extraction process; anti-inflammatory; antibacterial; growth promoting effect