基于免疫信息学设计猪流行性腹泻病毒S蛋白的通用多表位疫苗

doi:10.16431/j.cnki.1671-7236.2025.11.033

Abstract

Abstract: 【Objective】 The aim of this study was to design a universal multi-epitope vaccine for the prevention and control of Porcine epidemic diarrhea virus (PEDV) infections that could broadly respond to the current prevalent strains of PEDV in the country and potential new variants in the future. 【Method】 S protein of domestic PEDV epidemic strains was analyzed by genetic evolution and similarity comparison,and the conserved sequence was screened out to predict its antigenicity.Immunoinformatics tools such as IEDB,DTU Health Tech,and ABCpred were used to screen T lymphocyte and B lymphocyte epitopes.By connecting them in series through flexible linkers,a multi-epitope vaccine was constructed.Subsequent predictions on the antigenicity,allergenicity,toxicity,N-glycosylation sites,physicochemical properties,secondary structure,and tertiary structure of the vaccine were conducted.The immune response induced by the vaccine was evaluated through immunological simulation. 【Result】 Four conserved antigenic sequences were successfully screened from the S protein,and three cytotoxic T lymphocyte (CTL) epitopes,five helper T lymphocyte(HTL) epitopes,and six linear B cell epitopes were identified.The epitopes were recombinantly constructed as a multi-epitope vaccine,rPEDV-S,with a molecular mass of 33.53 ku,which exhibited hydrophilicity,had two potential N-glycosylation sites,and was non-allergenic and non-toxic.In the secondary structure of rPEDV-S,alpha helix,beta sheet and random coil accounted for 32.71%,14.02% and 53.27%,respectively.The tertiary structure analysis showed 95.3% of residues in Ramachandran favorable region with a Z-score of －5.30.The immunological simulation results showed that rPEDV-S could induce strong T cell and B cell immune responses. 【Conclusion】 This study successfully designed a multi-epitope vaccine rPEDV-S,which could stimulate a strong immune response,providing a new research approach and candidate vaccine for addressing the novel PEDV variant strains in the country.

Key words: Porcine epidemic diarrhea virus (PEDV); immunoinformatics; S protein; multi-epitope vaccine

CLC Number:

S852.65

XU Dawei, ZHU Qi, CHENG Anqi, CHEN Yushan, LIU Yuming, LI Bing. Design of a Universal Multi-epitope Vaccine for Porcine Epidemic Diarrhea Virus S Protein Based on Immunoinformatics[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5381-5392.

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Design of a Universal Multi-epitope Vaccine for Porcine Epidemic Diarrhea Virus S Protein Based on Immunoinformatics

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