枯草芽孢杆菌-地衣芽孢杆菌复合菌剂对泌乳奶牛饲料消化利用及生产性能的影响

doi:10.16431/j.cnki.1671-7236.2025.11.008

Abstract

Abstract: 【Objective】 This study aimed to investigate the effects of dietary supplementation with a compound bacterial agent containing Bacillus subtilis and Bacillus licheniformis on rumen fermentation,nutrient digestibility and production performance in lactating dairy cows. 【Method】 A total of 564 Holstein dairy cows with similar parity,milk production and lactation age were selected.They were randomly divided into the control group (CON group,n＝247) and the test group supplemented with 9.6×10⁹ CFU/(cow·d) of the combined bacterial agent (BAC group,n＝317).The trial period lasted for 11 weeks,with a 2-week pre-feeding period and a 9-week main trial period.Daily milk production of each cow and the daily feed intake of each group were recorded.Feed samples were collected every week,and the contents of conventional nutritional components such as dry matter,organic matter,crude protein,and crude fat were determined.At the 4th and 9th weeks of the experiment,milk,rumen fluid and fecal samples were collected. And the milk components,rumen fluid pH,ammonia nitrogen,microbial protein,enzyme activity and volatile fatty acid contents,and the contents of dry matter,organic matter,crude protein,crude fat,and other routine nutritional components as well as the acid-insoluble ash content in the fecal samples were determined. 【Result】 After supplementing the dairy cow feed with compound bacterial agent containing Bacillus subtilis and Bacillus licheniformis,there was a decreasing trend in the ammonia nitrogen concentration in the rumen fluid (P＝0.07),and there was an increasing trend in the carboxymethyl cellulase activity (P＝0.07).Compared with CON group,at the 9th week of the experiment,the apparent digestibility of crude protein and crude fat of dairy cows in BAC group increased by 4.18% and 3.91% respectively (P＜0.05),and the apparent digestibility of neutral detergent fiber showed an upward trend (P＝0.06).During the 8th and 9th weeks of the experiment,the milk production of BAC group cows increased by 0.51% and 0.77% respectively (P＜0.05),and the feed conversion rate (milk production/dry matter intake) of BAC group improved by 9.76% in the 9th week of the experiment (P＜0.05). 【Conclusion】 Dietary supplementation with compound bacterial agent containing Bacillus subtilis and Bacillus licheniformis (9.6×10⁹ CFU/(cow·d)) could increase the apparent digestibility of dietary nutrients and feed conversion rate,reduce the ammonia nitrogen concentration in rumen fluid,and improve production performance.

Key words: compound bacteria agent; lactating dairy cow; production performance; apparent digestibility; ruminal fermentation

CLC Number:

S816

CHENG Fengqi, MA Lu, BU Dengpan, ZHAN Tengfei, XU Lianbin. Effect of Bacillus subtilis-Bacillus licheniformis Compound Bacteria Agent on the Feed Digestion,Utilization and Production Performance of Lactating Dairy Cows[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5100-5109.

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Effect of Bacillus subtilis-Bacillus licheniformis Compound Bacteria Agent on the Feed Digestion,Utilization and Production Performance of Lactating Dairy Cows

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