Abstract: RNA sequencing (RNA-Seq) technology is a powerful tool that reveals various complex biological phenomena by analyzing the transcriptional levels of genes and regulatory elements within cells.It has a wide range of applications in fields such as agricultural breeding,variety resources,nutrition metabolism and medicine.Ribosomal RNA (rRNA) accounts for over 80% of the total RNA in cells. Direct RNA-Seq library sequencing of samples yields data in which more than 90% of the sequences from rRNA,making it difficult to detect various low abundance RNAs.rRNA reduction technology can significantly improve the gene detection efficiency and sensitivity of RNA-Seq technology,and can quantitatively detect low abundance non-coding RNA,providing more accurate data support for revealing some complex biological mechanisms.However,the role of this technology in RNA-Seq library construction has often not been given due attention,resulting in some sequencing data not reflecting the true value of the sample.The authors provide an overview of several rRNA reduction strategies in RNA-Seq,including Olig dT magnetic bead capture method,“pull-out” method,RNase H selective reduction method,and thermostable duplex-specific nuclease (DSN) selective method, non random primer reverse transcription method,and RNA blocking primer methods,etc.The advantages and disadvantages of each strategy are compared,and intended to select the most suitable ribosomal RNA reduction strategy for researchers employing RNA-Seq technology,provide support and reference for obtaining research data that is closest to the true value of the sample,and enable new technologies to better serve scientific research and technological innovation.

Key words: RNA-Seq; rRNA; strategies reduction