非编码小RNA FrnS基因缺失对鼠伤寒沙门菌致病性的影响

doi:10.16431/j.cnki.1671-7236.2025.10.036

Abstract

Abstract: 【Objective】 This study aimed to explore the effect of FnrS gene on the pathogenicity of Salmonella Typhimurium by constructing non-coding small RNA (sRNA) FnrS gene deletion strain.【Method】 The λ-Red homologous recombination technology was used to construct the FnrS gene deletion strain ΔFnrS of Salmonella Typhimurium,and its genetic stability,growth curve and biochemical characteristics were determined.The effect of FnrS gene on the pathogenicity of Salmonella Typhimurium was analyzed by detecting the median lethal dose (LD₅₀) of the strain on mice,the bacterial load of the tissue and the pathological tissue sections.【Result】 The FnrS gene deletion strain ΔFnrS was successfully constructed and it could be stably inherited.The growth curve and biochemical test results showed that the deletion of FnrS gene did not affect the growth ability and biochemical characteristics of Salmonella Typhimurium.The results of the pathogenicity test in mice showed that the LD₅₀ of deletion strain ΔFnrS for mice was 1.06×10⁵ CFU，which was significantly higher than that of parental and complement strains (P＜0.05).There was no significant difference in the survival rate of mice between deletion strain ΔFnrS and parental strain (P＞0.05).At 7 and 9 days after infection with deletion strain ΔFnrS,the bacterial load in the liver of mice was significantly lower than that of parental and complement strains (P＜0.05).At 5，7 and 9 days after infection，the bacterial load in the spleen of mice was significantly lower than that of parental and complement strains (P＜0.05).【Conclusion】 The deletion of FnrS gene reduced the pathogenicity of Salmonella Typhimurium.This results laid a foundation for the further research of the pathogenic mechanism of Salmonella Typhimurium.

Key words: Salmonella Typhimurium; sRNA; FnrS gene; pathogenicity

CLC Number:

S852.61

NING Chengcheng, TIAN Lulu, FENG Kai, CHEN Ying, AI Jiawuyi, LIU Xuwei, YANG Liwei, ZHANG Zaichao. Effect of sRNA FrnS Gene Deletion on the Pathogenicity of Salmonella Typhimurium[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4912-4919.

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Effect of sRNA FrnS Gene Deletion on the Pathogenicity of Salmonella Typhimurium

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